Browsing by Author "JORDAN, M"

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    EFFECTS OF NITROGENOUS BASES ON ROOT-FORMATION OF HYPOCOTYLS FROM PRUNUS-AVIUM L MERICIER AND BING GROWN-INVITRO
    (1982) JORDAN, M; ITURRIAGA, L; FEUCHT, W
    The rooting capacity of hypocotyl segments of sweet cherry cv. Mericier and Bing was investigated. NAA [naphthaleneacetic acid] (1 mg/l) and kinetin (0.1 mg/l) stimulated root induction (''Mericier'' 82.9%, ''Bing'' 44.4%). Without kinetin rooting was reduced to 37.1% in ''Mericier'' and to nil in ''Bing''. Purine and pyrimidine bases enhanced root formation. Cytosine proved optimal (''Mericier'' 80%, ''Bing'' 55.6%).
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    INHIBITION OF ROOT-FORMATION IN PRUNUS-AVIUM HYPOCOTYLS BY CHLOROGENIC ACID INVITRO
    (1980) JORDAN, M; ITTURRIAGA, L; FEUCHT, W
    Etiolated hypocotyls of difficult to root P. avium were grown in vitro and were induced to form adventitious roots on a modified Murashige and Skoog medium. Naphthalene acetic acid at 1.0 mg/l together with furfurylaminopurine at 0.1 mg/l were most active (97.5%) in promoting roots. Chlorogenic acid supplied to the media at different concentrations reduced root formation and callus growth; the content of total phenolics was also reduced.
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    INVITRO REGENERATION OF PROSOPIS TAMARUGO PHIL AND PROSOPIS CHILENSIS (MOL) STUNTZ FROM NODAL SECTIONS
    (1985) JORDAN, M; BALBAO, O
    Nodal segments of Prosopis chilensis and P. tamarugo from nine month-old plants were investigated as regards their rooting capacity and asexual propagation in vitro. High levels of naphthaleneacetic acid (5-10 mg/l) were most efficient for rooting and regeneration of plantlets in both species, although the inclusion of kinetin and/or gibberellic acid (0.01 mg/l) favoured axillary bud development after root formation. Rhizogenesis was observed after 3-4 weeks, consisting of one or more roots per node independent of preliminary callus formation. Rooting responses and plantlet regeneration were always better in P. chilensis than in P. tamarugo, under the same hormonal treatment.
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    INVITRO-PROPAGATION STUDIES OF 3 PROSOPIS SPECIES (PROSOPIS-ALBA, PROSOPIS-CHILENSIS AND PROSOPIS-TAMARUGO) THROUGH SHOOT-TIP CULTURE
    (1985) JORDAN, M; PEDRAZA, J; GOREUX, A
    Plantlets of Prosopis chilensis and P. alba were regenerated in vitro through shoot-tip culture. Shoot-tips of both species initiated growth, showed multiple-shoot formation and developed roots within 4 weeks when cultivated in a nutrient medium containing the phytohormones naphthalene acetic acid (NAA), benzyladenine (BA), and gibberellic acid (GA3), or after subculture using casein hydrolysate and indolebutyric acid (IBA) as single hormone. Under the same conditions the 3-4 uppermost axillary buds of shoot sections set in culture initiated growth and when fragmented and subcultured in presence of IBA regenerated plantlets.
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    ISOLATION, CULTURE AND FUSION OF CARICA-CANDAMARCENCIS AND CARICA-PAPAYA PROTOPLASTS
    (1986) JORDAN, M; CIUDAD, G; ROJAS, ML; VALVERDE, F
    Protoplasts were isolated from two sexually incompatible Caricaceae (Carica candamarcensis and C. papaya) edible species. Preplasmolysis and protoplast yield were established in response to enzyme concentration, digesting temperatures, nutrient media and tissue source. Fused protoplasts of both species as well as first steps in cell cluster formation were observed.
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    MULTIPLE SHOOT FORMATION AND RHIZOGENESIS FROM CHERIMOLA (ANNONA-CHERIMOLA L) HYPOCOTYLS AND PETIOLE EXPLANTS
    (1988) JORDAN, M
    Multiple shoot formation and their growth in Annona cherimola Mill. cv. Concha Lisa was attained along hypocotyl sections (3-4 mm long) and on petioles with a leaf portion. Adventitous shoot formation occurs directly, without undergoing the callus phase, in both types of explants; in petioles this also occurs in the foliar portion. Calli formed at the cut ends preceded rhizogenesis in a few cases only. Rhizogenesis and production of plantlets was obtained in two treatments: the first sub-culturing buds isolated from hypocotyl sections in presence of NAA; the second using longer hypocotyl sections (25 mm) which organized shoots and roots simultaneously in the distal and proximal regions.
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    POTENTIALITIES OF CELL AND CALLUS-TISSUE CULTURE TO REGENERATE 2 MESQUITE SPECIES (PROSOPIS-TAMARUGO AND PROSOPIS-CHILENSIS)
    (1987) JORDAN, M; CORTES, I; GOREUX, A
    Morphogenetic events in Prosopis tamarugo and P. chilensis calli and cell suspensions were studied in in vitro conditions. Under several growth conditions tested a generalized browning effect in tissues were observed. This effect was partially reduced by the use of cysteine in media. In P. tamarugo, shoot-like structures developed from calli after several subcultures. In P. chilensis, cell suspensions were able to form cell clusters and later on somatic embryoids. At present morphogenetic responses observed in both species occurred in a low frequency.
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    PROMOTION OF ANNONA CHERIMOLA INVITRO SHOOT MORPHOGENESIS AS INFLUENCED BY ANTIOXIDANTS
    (1991) JORDAN, M; ITURRIAGA, L; ROVERARO, C; GOREUX, A
    Nodal sections, internodes, and zygotic embryos of Annona cherimola 'Concha Lisa' were cultivated in vitro in several phytohormone combinations to trigger morphogenesis. De novo multiple shoot formation was the most common response. Various antioxidant compounds, especially PVP, ascorbic acid, and glutathione enhanced this effect and reduced browning, while DIECA and aminooxyacetic acid were less effective in the concentrations tested. Citric acid and cysteine were not beneficial. Combinations of PVP with casein hydrolysate in presence of NAA 0.5 mg/l plus BA 2.0 mg/l were the best blends to promote shoot formation and yielded the highest number of shoots per explant.
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    SHOOT ORGANOGENESIS IN TISSUE-CULTURE OF DRIMYS-WINTERI
    (1981) JORDAN, M; CORTES, I
    Calluses from stem explants of D. winteri Forst. (Winteraceae) initiated shoot organogenesis in vitro in hormone-containing media under light exposure. Shoots formed from callus-derived outgrowths and from the superficial callus layers of the explants. Light inhibited callus initiation but promoted growth of established cultures. Root organogenesis did not occur under any of various hormonal conditions. Active synthesis of anthocyanins was observed in morphogenetic tissue. [Plant tissue culture, its limitations and potential use in forest improvement was recently discussed. D. winteri Forst. (Winteraceae) is a representative tree species of the evergreen forest in southern Chile (40.degree. S latitude). As a fast growing species, it might become an alternative source of wood in the near future.].