MULTIPLE SHOOT FORMATION AND RHIZOGENESIS FROM CHERIMOLA (ANNONA-CHERIMOLA L) HYPOCOTYLS AND PETIOLE EXPLANTS
Abstract
Multiple shoot formation and their growth in Annona cherimola Mill. cv. Concha Lisa was attained along hypocotyl sections (3-4 mm long) and on petioles with a leaf portion. Adventitous shoot formation occurs directly, without undergoing the callus phase, in both types of explants; in petioles this also occurs in the foliar portion. Calli formed at the cut ends preceded rhizogenesis in a few cases only. Rhizogenesis and production of plantlets was obtained in two treatments: the first sub-culturing buds isolated from hypocotyl sections in presence of NAA; the second using longer hypocotyl sections (25 mm) which organized shoots and roots simultaneously in the distal and proximal regions.