INVITRO-PROPAGATION STUDIES OF 3 PROSOPIS SPECIES (PROSOPIS-ALBA, PROSOPIS-CHILENSIS AND PROSOPIS-TAMARUGO) THROUGH SHOOT-TIP CULTURE
No Thumbnail Available
Date
1985
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Plantlets of Prosopis chilensis and P. alba were regenerated in vitro through shoot-tip culture. Shoot-tips of both species initiated growth, showed multiple-shoot formation and developed roots within 4 weeks when cultivated in a nutrient medium containing the phytohormones naphthalene acetic acid (NAA), benzyladenine (BA), and gibberellic acid (GA3), or after subculture using casein hydrolysate and indolebutyric acid (IBA) as single hormone. Under the same conditions the 3-4 uppermost axillary buds of shoot sections set in culture initiated growth and when fragmented and subcultured in presence of IBA regenerated plantlets.