IMMUNOGENIC CAPACITY OF RIBOSOMES OF SALMONELLA-TYPHI INTERFERED WITH BY A FLAGELLIN-LIKE MATERIAL CONTAMINANT

COFRE, G; CALDERON, I; MORA, GC

IMMUNOGENIC CAPACITY OF RIBOSOMES OF SALMONELLA-TYPHI INTERFERED WITH BY A FLAGELLIN-LIKE MATERIAL CONTAMINANT

Date

1978

Authors

COFRE, G

CALDERON, I

MORA, GC

Abstract

The double-immunodiffusion technique and sodium dodecyl sulfate-polyacrylamide electrophoresis were used to demonstrate the presence of flagellin-like material strongly attached to ribosomes of S. typhi Ty 2. This flagellin-like material contaminating the ribosome preparation interfers with the induction of [rabbit] antiribosome serum, promoting the formation of antisera reacting either only with flagellin or in some cases with flagellin and ribosomes, but giving a very weak reaction with the latter. The interference is also observed when purified ribosomes from a nonflagellated mutant of S. typhi (S. typhi O-901) mixed with purified S. typhi Ty 2 flagellin are utilized as antigens. The antirosome sera obtained with ribosomes from S. typhi O-901 have a considerably higher titer than those that are interfered with. These sera were able to react with ribosomes obtained from several related species and did not react with flagella-derived flagellin of S. typhi Ty 2.