Fanconi anemia lymphocytes

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dc.contributor.authorPincheira, J
dc.contributor.authorBravo, M
dc.contributor.authorSantos, MJ
dc.contributor.authorde la Torre, C
dc.contributor.authorLópez-Sáez, JF
dc.date.accessioned2025-01-21T01:30:58Z
dc.date.available2025-01-21T01:30:58Z
dc.date.issued2001
dc.description.abstractThe high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells,
dc.description.abstractThe effect of 100 muM DL-alpha -tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G(2) repair was prevented by 2.5 mM caffeine (G(2) unrepaired damage). In addition, the effect of this antioxidant on G(2) duration and the efficiency of G(2) repair was also evaluated in the sample.
dc.description.abstractalpha -Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G(2) repair conditions) and the duration of G(2) in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).
dc.description.abstractThe efficiency of the G(2) repair process (G(2)R rate) defined as the ratio between the percentage of chromatid breaks repaired in G(2) and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G(2)R rate, both in FA cells and controls.
dc.description.abstractThese results suggest that an increased oxidative damage and a lower G(2) repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells. (C) 2001 Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.issn0921-8777
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96947
dc.identifier.wosidWOS:000166245900002
dc.issue.numero4
dc.language.isoen
dc.pagina.final271
dc.pagina.inicio265
dc.revistaMutation research-dna repair
dc.rightsacceso restringido
dc.subjectfanconi anemia
dc.subjectalpha-tocopherol
dc.subjectchromatid breaks
dc.subjectG(2) repair
dc.subjectlymphocytes
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFanconi anemia lymphocytes
dc.typeartículo
dc.volumen461
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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