Fanconi anemia lymphocytes
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Date
2001
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Abstract
The high frequency of chromosomal breaks in Fanconi anemia (FA) lymphocytes has been related to the increased oxidative damage shown by these cells,
The effect of 100 muM DL-alpha -tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G(2) repair was prevented by 2.5 mM caffeine (G(2) unrepaired damage). In addition, the effect of this antioxidant on G(2) duration and the efficiency of G(2) repair was also evaluated in the sample.
alpha -Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G(2) repair conditions) and the duration of G(2) in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).
The efficiency of the G(2) repair process (G(2)R rate) defined as the ratio between the percentage of chromatid breaks repaired in G(2) and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G(2)R rate, both in FA cells and controls.
These results suggest that an increased oxidative damage and a lower G(2) repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells. (C) 2001 Elsevier Science B.V. All rights reserved.
The effect of 100 muM DL-alpha -tocopherol (Vitamin E) on the level of chromosomal damage in mitosis was studied in lymphocytes from five FA patients and from age matched controls, both under basal conditions and when G(2) repair was prevented by 2.5 mM caffeine (G(2) unrepaired damage). In addition, the effect of this antioxidant on G(2) duration and the efficiency of G(2) repair was also evaluated in the sample.
alpha -Tocopherol (AT) decreased the frequency of chromosomal damage (under basal and inhibited G(2) repair conditions) and the duration of G(2) in FA cells. This antioxidant protective effect, expressed as the decrease in chromatid breaks, was greater in FA cells (50.8%) than in controls (25%).
The efficiency of the G(2) repair process (G(2)R rate) defined as the ratio between the percentage of chromatid breaks repaired in G(2) and the duration of this cell cycle phase was lesser in FA cells (10.6) than in controls (22.6). AT treatment slightly increased this G(2)R rate, both in FA cells and controls.
These results suggest that an increased oxidative damage and a lower G(2) repair rate may be simultaneously involved in the high frequency of chromatid damage detected in FA cells. (C) 2001 Elsevier Science B.V. All rights reserved.
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Keywords
fanconi anemia, alpha-tocopherol, chromatid breaks, G(2) repair, lymphocytes