STRUCTURE OF THE EUKARYOTIC GENOME - A UNIQUE PSEUDOGENE LACKING INTRONS AND POLY-A TAIL AS A MEMBER OF THE HUMAN BETA-TUBULIN GENE FAMILY
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Date
1982
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Abstract
From a human gene bank constructed in the vector phage Charon 4A, 14 clones carrying complementary sequences to .alpha. and .beta. tubulin chicken c[complementary]DNA were isolated. A preliminary structural analysis of some of the .beta. clones, based on restriction digests followed by Southern hybridization, allowed us to select clone .beta.9 as the one containing a functional gene. After further analysis this clone was proved to contain a pseudogene. The coding sequence is altered by 4 stop codons, 6 short delections and 2 1-base insertions. These changes prevent any correct translation. The pseudogene seems to preserve some vestigial control regions and does not contain introns. No in vitro RNA synthesis directed by this clone was detected.