Development of Electroanalytical Methods Using Different Electrodes for the Determination of Progesterone (P4) in Pharmaceutical Formulations and Human, Cow and Goat Milk
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Date
2023
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Abstract
For the determination of progesterone (P4) electroanalytical studies were performed using mercury electrode (HMDE) and screen-printed boron-doped diamond electrode (BDD-SPE). The effects of pH, supporting electrolyte, accumulation potential and time (E-acc, t(acc)) were studied in both systems. The optimum conditions were: i) HMDE: pH 7,0; -0,1 V and t(acc): 40 s, ii) BDD-SPE: 0,5 mol L-1 H2S ; E-acc: 0,0 V and t(acc): 120 s. Under these conditions, the detection limit was 3.1 mu g L-1 for HMDE and 45.6 mu g L-1 for BDD-SPE. Both methods were validated and applied in the P4 determination on pharmaceutical formulations and milk samples.
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Keywords
Progesterone (P4), HMDE, BDD-SPE, Milk samples, Pharmaceutical formulations (Progendo (R), Progest (R), Progeffik (R))