RESPIRATORY SYNCYTIAL VIRUS DETECTION BY DOT BLOT HYBRIDIZATION WITH A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE

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dc.contributor.authorHERNANDEZ, O
dc.contributor.authorFERNANDEZ, J
dc.contributor.authorVALENZUELA, S
dc.contributor.authorSANDINO, AM
dc.contributor.authorPIZARRO, J
dc.contributor.authorVASQUEZ, M
dc.contributor.authorYUDELEVICH, A
dc.contributor.authorSPENCER, E
dc.date.accessioned2025-01-23T19:22:06Z
dc.date.available2025-01-23T19:22:06Z
dc.date.issued1992
dc.description.abstractA synthetic oligodeoxynucleotide corresponding to a region of the nucleocapside gene (N) of respiratory syncytial virus (RSV), was used as a DNA probe to develop a nonradioactive hybridization assay for the detection of RSV. The probe was labeled by incorporation of biotin-7-dATP to the 3' end by a reaction catalyzed by terminal deoxynucleotydil transferase.
dc.description.abstractThe dot blot hybridization assay was found to be specific for RSV when tested against RSV isolates (subgroups A and B) obtained from cell cultures and isolates of adenovirus, reovirus, rotavirus, and pararotavirus. The assay detected both RSV subgroups (A and B) without significant differences. The dot blot hybridization assay using the nonradioactive probe led to similar results to indirect immunofluorescence (IFI) when tested against a panel of 64 clinical samples from nasopharyngeal secretions of infants with clinical symptoms of respiratory disease. This assay may provide the basis for a rapid, simple, and inexpensive method for routine RSV diagnosis.
dc.fuente.origenWOS
dc.identifier.issn0146-6615
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98961
dc.identifier.wosidWOS:A1992JC61900002
dc.issue.numero3
dc.language.isoen
dc.pagina.final169
dc.pagina.inicio165
dc.revistaJournal of medical virology
dc.rightsacceso restringido
dc.subjectRESPIRATORY SYNCYTIAL VIRUS
dc.subjectNONRADIOACTIVE PROBE
dc.subjectNUCLEOCAPSIDE GENE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleRESPIRATORY SYNCYTIAL VIRUS DETECTION BY DOT BLOT HYBRIDIZATION WITH A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE
dc.typeartículo
dc.volumen37
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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