ROTAVIRUS DETECTION BY DOT BLOT HYBRIDIZATION ASSAY USING A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE

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dc.contributor.authorFERNANDEZ, J
dc.contributor.authorSANDINO, A
dc.contributor.authorYUDELEVICH, A
dc.contributor.authorAVENDANO, LF
dc.contributor.authorVENEGAS, A
dc.contributor.authorHINRICHSEN, V
dc.contributor.authorSPENCER, E
dc.date.accessioned2025-01-23T19:22:18Z
dc.date.available2025-01-23T19:22:18Z
dc.date.issued1992
dc.description.abstractA synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus. was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.
dc.fuente.origenWOS
dc.identifier.issn0950-2688
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99010
dc.identifier.wosidWOS:A1992HK03200018
dc.issue.numero1
dc.language.isoen
dc.pagina.final184
dc.pagina.inicio175
dc.revistaEpidemiology and infection
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleROTAVIRUS DETECTION BY DOT BLOT HYBRIDIZATION ASSAY USING A NONRADIOACTIVE SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBE
dc.typeartículo
dc.volumen108
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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