INHIBITION OF ATRIAL-NATRIURETIC-PEPTIDE INDUCED NATRIURESIS BY PLASMA HYDROLYSATES CONTAINING PEPSANURIN

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dc.contributor.authorBORIC, MP
dc.contributor.authorCROXATTO, HR
dc.contributor.authorALBERTINI, R
dc.contributor.authorROBLERO, JS
dc.date.accessioned2025-01-23T19:22:20Z
dc.date.available2025-01-23T19:22:20Z
dc.date.issued1992
dc.description.abstractThe specificity of antidiuretic actions of pepsanurin, a peptidic fraction obtained by pepsin hydrolysis of plasma, was studied in anesthetized rats and in isolated perfused rat kidneys. Pepsanurin was obtained from fresh dialyzed human plasma digested with pepsin (2,400 units/ml, 18 hours at 37-degrees-C, pH 2.5), deproteinized (10 minutes at 80-degrees-C), and centrifuged. In the rat, intraperitoneal injections of pepsanurin (0.5 ml/100 g body wt) significantly inhibited the effects of an intravenous bolus of atrial natriuretic peptide (ANP) (0.5-mu-g) on water, sodium, and potassium excretion without altering systemic blood pressure. In addition, pepsanurin abolished the peak in glomerular filtration rate and reduced the ANP-induced rise in fractional sodium excretion. Pepsanurin also inhibited the natriuretic effects of amiloride (10-mu-g/100 g body wt i.v.) without changing glomerular filtration rate, but it did not inhibit the potassium-retaining effect of amiloride. In contrast, pepsanurin had no effect on basal urinary excretion, and it did not affect the diuretic response induced by furosemide (doses of 25, 50, or 100-mu-g i.v.). Control peptidic hydrolysates prepared from human plasma preincubated 48 hours at 37-degrees-C (PIPH), bovine albumin (BSAH), or human albumin did not inhibit ANP, amiloride, or furosemide. In perfused kidneys, pepsanurin significantly and reversibly reduced sodium and water excretion. Furthermore, pepsanurin, but not PIPH or BSAH, blocked the natriuretic and diuretic effects of ANP. These results support the existence of a specific plasma substrate able to release a peptide or peptides that counteract distal tubule diuresis and natriuresis by an intrarenal mechanism.
dc.fuente.origenWOS
dc.identifier.eissn1524-4563
dc.identifier.issn0194-911X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99016
dc.identifier.wosidWOS:A1992HD54300048
dc.issue.numero2
dc.language.isoen
dc.pagina.final250
dc.pagina.inicio243
dc.revistaHypertension
dc.rightsacceso restringido
dc.subjectPEPTIDES
dc.subjectATRIAL NATRIURETIC PEPTIDES
dc.subjectAMILORIDE
dc.subjectFUROSEMIDE
dc.subjectKIDNEY
dc.subjectANTIDIURETICS
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleINHIBITION OF ATRIAL-NATRIURETIC-PEPTIDE INDUCED NATRIURESIS BY PLASMA HYDROLYSATES CONTAINING PEPSANURIN
dc.typeartículo
dc.volumen19
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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