ASSEMBLY AND DISASSEMBLY OF AXONAL MICROTUBULES OF THE TOAD XENOPUS-LAEVIS UNDER THE EFFECT OF TEMPERATURE

dc.contributor.authorALVAREZ, J
dc.contributor.authorFADIC, R
dc.date.accessioned2025-01-23T19:21:59Z
dc.date.available2025-01-23T19:21:59Z
dc.date.issued1992
dc.description.abstractIn toads Xenopus laevis living at 11-degrees (winter), the microtubular density of 4-mum myelinated axons of lumbosacral nerves was assessed with the electron microscope. In controls, the density was 11.2 microtubules/mum2. In nerves incubated at 0-degrees, microtubules decreased following a simple exponential curve with a half time of 4.7 min (k = 0.149 min-1); residual microtubules were 4.5%. After rewarming, the full complement of microtubules reappeared within 60 min. In steady state, the microtubular density exhibited a linear relationship with temperature (range: 0-22-degrees; slope 0.94 microtubules/mum2 per degree; r, 0.96). After heating the nerve by 11-degrees above the physiological temperature, microtubules increased by 83%, whereby the pool of unpolymerized tubulin was at least 2.7 mg/ml of axoplasm. A seasonal variation of the microtubular density was observed which accorded with the environmental temperature. The macroscopic kinetics of microtubule disassembly in the axoplasm is similar to that reported for purified tubulin but that of assembly is slower. Microtubules of peripheral axons of Xenopus are cold-labile and vary during the annual cycle.
dc.fuente.origenWOS
dc.identifier.issn0022-104X
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98929
dc.identifier.wosidWOS:A1992JZ32400004
dc.issue.numero3
dc.language.isoen
dc.pagina.final266
dc.pagina.inicio261
dc.revistaJournal of experimental zoology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleASSEMBLY AND DISASSEMBLY OF AXONAL MICROTUBULES OF THE TOAD XENOPUS-LAEVIS UNDER THE EFFECT OF TEMPERATURE
dc.typeartículo
dc.volumen264
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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