Lack of canonical activities of connexins in highly aggressive human prostate cancer cells

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dc.article.number97
dc.catalogadoraba
dc.contributor.authorAsencio Barría, Catalina Andrea
dc.contributor.authorVéliz García, Loreto Pamela
dc.contributor.authorFlores-Faúndez, Emilia
dc.contributor.authorAzócar, Lorena
dc.contributor.authorEcheverría, Carolina E.
dc.contributor.authorTorres Estay, Verónica
dc.contributor.authorOrellana, Viviana
dc.contributor.authorRamírez Santelices, Catalina
dc.contributor.authorSotomayor Fahrenkrog, Paula Camila Stefanía
dc.contributor.authorCancino, Jorge
dc.contributor.authorKerr, Bredford
dc.contributor.authorFernandez-Olivares, Ainoa
dc.contributor.authorRetamal, Mauricio A.
dc.contributor.authorSáez, Juan C.
dc.contributor.authorGodoy, Alejandro S.
dc.date.accessioned2024-12-30T13:34:46Z
dc.date.available2024-12-30T13:34:46Z
dc.date.issued2024
dc.date.updated2024-12-22T01:04:59Z
dc.description.abstractAbstract Connexins (Cxs) have the ability to form channels that allow the exchange of ions/metabolites between adjacent cells (gap junction channels, GJC) or between the intra- and extra-cellular compartments (hemichannels, HC). Cxs were initially classified as tumor suppressors. However, more recently, it has been shown that Cxs exert anti- and pro-tumorigenic effects depending on the cell and tissue context. In prostate cancer (PCa), the expression and functionality of Cxs remain highly controversial. Here, we analyzed the expression pattern of Cx26, Cx32, Cx37, Cx40, Cx43 and Cx45 in PCa cell lines with increasing levels of tumor aggressiveness (LNCaP < LNCaP-C4-2 < Du-145 < PC-3). In addition, GJ and HC activities were evaluated in the PCa cell lines using dye coupling and dye uptake assays, respectively. Lastly, the cellular localization of Cx26, Cx32, and Cx43 was analyzed in LNCaP and PC-3 cell lines using immunofluorescence analyses. Our results showed a positive association between the mRNA levels of Cx26, Cx37 and Cx45 and the degree of aggressiveness of PCa cells, a negative association in the case of Cx32 and Cx43, and no clear pattern for Cx40. At the protein level, a negative relationship between the expression of Cx26, Cx32 and Cx43 and the degree of aggressiveness of PCa cell lines was observed. No significant differences were observed for the expression of Cx37, Cx40, and Cx45 in PCa cell lines. At the functional level, only LNCaP cells showed moderate GJ activity and LNCaP and LNCaP-C4-2 cells showed HC activity. Immunofluorescence analyses confirmed that the majority of Cx26, Cx32, and Cx43 expression was localized in the cytoplasm of both LNCaP and PC3 cell lines. This data indicated that GJ and HC activities were moderately detected only in the less aggressive PCa cells, which suggest that Cxs expression in highly aggressive PCa cells could be associated to channel-independent roles.
dc.fechaingreso.objetodigital2024-12-19
dc.format.extent12 páginas
dc.fuente.origenAutoarchivo
dc.identifier.doi10.1186/s40659-024-00565-3
dc.identifier.issn0717-6287
dc.identifier.scopusid2-s2.0-85212414170
dc.identifier.urihttps://doi.org/10.1186/s40659-024-00565-3
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/89353
dc.identifier.wosidWOS:001381001300002
dc.information.autorucFacultad de Ciencias Biológicas; Asencio Barría, Catalina Andrea; S/I; 221029
dc.information.autorucFacultad de Ciencias Biológicas; Véliz García, Loreto Pamela; S/I; 95169
dc.information.autorucEscuela de Medicina; Sotomayor Fahrenkrog, Paula Camila Stefanía; S/I; 1083050
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final12
dc.pagina.inicio1
dc.revistaBiological Research
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectProstate cancer
dc.subjectConnexins
dc.subjectGap junctions
dc.subjectHemichannels
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleLack of canonical activities of connexins in highly aggressive human prostate cancer cells
dc.typeartículo
dc.volumen57
sipa.codpersvinculados221029
sipa.codpersvinculados95169
sipa.codpersvinculados1083050
sipa.indexScopus
sipa.indexWOS
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