Novel Type IIS-Based Library Assembly Technique for Developing Nanobodies Targeting IPNv VP2 Protein

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dc.article.number9350
dc.catalogadorpva
dc.contributor.authorPino-Belmar, Camila
dc.contributor.authorHimelreichs, Johanna
dc.contributor.authorDeride, Camila
dc.contributor.authorMatute Torres, Tamara Francisca
dc.contributor.authorNúñez Quijada, Isaac Natán
dc.contributor.authorCazaux, Severine Marianne
dc.contributor.authorFederici, Fernán
dc.contributor.authorMoreno-Mendieta, Karen
dc.contributor.authorSoto-Rauch, Genaro
dc.contributor.authorCastro, Joaquín
dc.contributor.authorFrenkel, Valentina
dc.contributor.authorHo, Joi-Hui
dc.contributor.authorAscencios, David
dc.contributor.authorSanhueza Teneo, Daniel
dc.contributor.authorMunizaga, José
dc.contributor.authorHaussmann, Denise
dc.contributor.authorRojas-Fernandez, Alejandro
dc.contributor.authorFigueroa Valverde, Jaime
dc.contributor.authorValenzuela-Nieto, Guillermo
dc.date.accessioned2025-10-01T19:05:12Z
dc.date.available2025-10-01T19:05:12Z
dc.date.issued2025
dc.description.abstractThe development of effective tools to combat viral diseases remains a major challenge for the aquaculture industry. Infectious pancreatic necrosis virus (IPNv) is one of the most devastating pathogens affecting salmonids, leading to high mortality rates and substantial economic losses worldwide. Here, we present a novel nanobody discovery pipeline based on a Type IIS restriction enzyme-driven library assembly method that enables the rapid generation of highly diverse nanobody repertoires. This streamlined approach not only shortens the time required for nanobody identification but also offers remarkable adaptability, allowing its application to virtually any protein target, including antigens from aquaculture pathogens and beyond. By integrating this strategy with density gradient–based enrichment and high-throughput screening, we successfully identified and validated a nanobody against the VP2 protein of IPNv, a key structural component essential for viral infectivity. These findings highlight the potential of this platform both as a versatile methodological advance in antibody engineering and as a practical foundation for developing innovative diagnostic and therapeutic tools. Ultimately, nanobodies generated through this pipeline could play a pivotal role in improving disease management and enhancing sustainability in aquaculture.
dc.fechaingreso.objetodigital2025-10-01
dc.format.extent20 páginas
dc.fuente.origenORCID
dc.identifier.doi10.3390/ijms26199350
dc.identifier.urihttps://doi.org/10.3390/ijms26199350
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105861
dc.information.autorucEscuela de Ingeniería; Matute Torres, Tamara Francisca; 0000-0002-0486-985X; 186256
dc.information.autorucEscuela de Ingeniería; Núñez Quijada, Isaac Natán; 0000-0002-4439-3745; 186208
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Cazaux, Severine Marianne; S/I; 1158443
dc.information.autorucFacultad de Ciencias Biológicas; Federici, Fernán; 0000-0001-9200-5383; 123357
dc.information.autorucInstituto de Ingeniería Biológica y Médica; Federici, Fernán; 0000-0001-9200-5383; 123357
dc.issue.numero19
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherMDPI
dc.revistaInternational Journal of Molecular Sciences
dc.rightsacceso abierto
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectIPNv
dc.subjectSingle domain antibodies
dc.subjectVP2
dc.subjectType IIS-based assembly methods
dc.subject.ddc570
dc.subject.deweyBiologíaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNovel Type IIS-Based Library Assembly Technique for Developing Nanobodies Targeting IPNv VP2 Protein
dc.typeartículo
dc.volumen26
sipa.codpersvinculados186256
sipa.codpersvinculados186208
sipa.codpersvinculados1158443
sipa.codpersvinculados123357
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