Electrophysiological characterization of nicotinic acetylcholine receptors in cat petrosal ganglion neurons in culture

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dc.contributor.authorVaras, R
dc.contributor.authorValdés, V
dc.contributor.authorIturriaga-Vásquez, P
dc.contributor.authorCassels, BK
dc.contributor.authorIturriaga, R
dc.contributor.authorAlcayaga, J
dc.date.accessioned2025-01-21T01:06:22Z
dc.date.available2025-01-21T01:06:22Z
dc.date.issued2006
dc.description.abstractPetrosal ganglion neurons are depolarized and fire action potentials in response to acetylcholine and nicotine. However, little is known about the subtype(s) of nicotinic acetylcholine receptors involved, although alpha 4 and alpha 7 subunits have been identified in petrosal ganglion neurons. Cytisine, an alkaloid unrelated to nicotine, and its bromo derivatives are agonists exhibiting different affinities, potencies and efficacies at nicotinic acetylcholine receptors containing a4 or 0 subunits. To characterize the receptors involved, we studied the effects of these agonists and the nicotinic acetylcholine receptor antagonists hexamethonium and alpha-bungarotoxin in isolated petrosal ganglion neurons. Petrosal ganglia were excised from anesthetized cats and cultured for up to 16 days. Using patch-clamp technique, we recorded whole-cell currents evoked by 5-10 s applications of acetylcholine, cytisine or its bromo derivatives. Agonists and antagonists were applied by gravity from a pipette near the neuron surface. Neurons responded to acetylcholine, cytisine, 3-bromocytisine and 5-bromocytisine with fast inward currents that desensitized during application of the stimuli and were reversibly blocked by 1 mu M hexamethoniurn or 10 nM alpha-bungarotoxin. The order of potency of the agonists was 3-bromocytisine >> acetylcholine congruent to cytisine >> 5-bromocytisine, suggesting that homomeric alpha 7 neuronal nicotinic receptors predominate in cat petrosal ganglion neurons in culture. (c) 2005 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.brainres.2005.12.006
dc.identifier.eissn1872-6240
dc.identifier.issn0006-8993
dc.identifier.urihttps://doi.org/10.1016/j.brainres.2005.12.006
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96144
dc.identifier.wosidWOS:000236051900011
dc.issue.numero1
dc.language.isoen
dc.pagina.final78
dc.pagina.inicio72
dc.revistaBrain research
dc.rightsacceso restringido
dc.subjectacetylcholine subunit
dc.subjectalpha 7 subunit
dc.subjectcarotid body
dc.subjectcytisine
dc.subjectnicotinic receptor
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleElectrophysiological characterization of nicotinic acetylcholine receptors in cat petrosal ganglion neurons in culture
dc.typeartículo
dc.volumen1072
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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