Fertilizing characteristics of bovine sperm with flattened or indented acrosomes

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dc.contributor.authorThundathil, J
dc.contributor.authorPalomino, J
dc.contributor.authorBarth, A
dc.contributor.authorMapletoft, R
dc.contributor.authorBarros, C
dc.date.accessioned2025-01-21T01:30:42Z
dc.date.available2025-01-21T01:30:42Z
dc.date.issued2001
dc.description.abstractFrozen semen from a control bull (C: 89% morphologically normal sperm) and two bulls with acrosomal defects (K1: 92% flattened acrosomes; K2: 82% indented acrosomes) were used to investigate the fertilizing ability of bull sperm with flattened or indented acrosomes. In experiment 1, frozen-thawed sperm were evaluated for acrosomal integrity with fluorescent microscopy. In experiment 2, proteolytic activity of the acrosomal contents of sperm was evaluated through a gelatin digestion assay. In experiment 3, an IVF test system was used to determine the ability of sperm with flattened or indented acrosomes to bind to bovine oocytes and penetrate the zona pellucida. In experiment 4, IVM zona-free bovine oocytes (ZFO) were fertilized and examined to evaluate sperm chromatin decondensation. In experiment 1, bulls K1 and K2 had a lower proportion of sperm with intact acrosomes (0 and 13.6 +/- 4.5%, respectively) than bull C (30.2 +/- 5.6%) after 2 h of incubation. In experiment 2, the proportion of sperm with proteolytic activity, as indicated by gelatin digestion around sperm heads, did not differ among bulls (C: 55%, n = 410; K1: 43%, n = 426; K2: 48%, n = 324). In experiment 3, a lower proportion of sperm with flattened (K1) or indented acrosomes (K2) bound to oocytes than sperm from the control bull, C. The percentage of zona penetrated (55%, n = 20; 13%, n = 23; 4%, n = 25) and the mean (+/-S.E.M.) number of sperm penetrating these zona pellucida (19.7 +/- 2.5; 6.9 +/- 1.0; and 2.6 +/- 0.5) was higher (P < 0.05) for bull C than for bulls K1 or K2, respectively. In experiment 4, the percentage of ZFO penetrated (95%, n = 20; 52%, n = 30; 30%, n = 33) and the mean (+/-S.E.M.) number of sperm with chromatin decondensation (7.8 +/- 1.6; 0.8 +/- 0.2; and 0.3 +/- 0.1) were also higher (P < 0.05) for the control bull, C than for bulls K1 or K2, respectively. Results suggest that although sperm with the flattened or indented acrosomes had a tendency to undergo spontaneous acrosome reaction on incubation after thawing, the proteolytic activity of the acrosomal contents appeared to be normal. Sperm with the flattened or indented acrosomes also appeared to have a reduced ability to fuse with oolemma as demonstrated by confocal microscopy. This would impair the ability to penetrate ooplasm and undergo sperm chromatin decondensation. (C) 2001 Elsevier Science B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.issn0378-4320
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96862
dc.identifier.wosidWOS:000171061900010
dc.issue.numero3-4
dc.language.isoen
dc.pagina.final243
dc.pagina.inicio231
dc.revistaAnimal reproduction science
dc.rightsacceso restringido
dc.subjectbull semen
dc.subjectacrosomal defects
dc.subjectacrosome reaction
dc.subjectinfertility
dc.subjectIVF
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFertilizing characteristics of bovine sperm with flattened or indented acrosomes
dc.typeartículo
dc.volumen67
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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