Statistical parametric maps of <SUP>18</SUP>F-FDG PET and 3-D autoradiography in the rat brain: a cross-validation study

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dc.contributor.authorPrieto, Elena
dc.contributor.authorCollantes, Maria
dc.contributor.authorDelgado, Mercedes
dc.contributor.authorJuri, Carlos
dc.contributor.authorGarcia-Garcia, Luis
dc.contributor.authorMolinet, Francisco
dc.contributor.authorFernandez-Valle, Maria E.
dc.contributor.authorPozo, Miguel A.
dc.contributor.authorGago, Belen
dc.contributor.authorMarti-Climent, Josep M.
dc.contributor.authorObeso, Jose A.
dc.contributor.authorPenuelas, Ivan
dc.date.accessioned2025-01-20T23:59:52Z
dc.date.available2025-01-20T23:59:52Z
dc.date.issued2011
dc.description.abstractPurpose Although specific positron emission tomography (PET) scanners have been developed for small animals, spatial resolution remains one of the most critical technical limitations, particularly in the evaluation of the rodent brain. The purpose of the present study was to examine the reliability of voxel-based statistical analysis (Statistical Parametric Mapping, SPM) applied to F-18-fluorodeoxyglucose (FDG) PET images of the rat brain, acquired on a small animal PET not specifically designed for rodents. The gold standard for the validation of the PET results was the autoradiography of the same animals acquired under the same physiological conditions, reconstructed as a 3-D volume and analysed using SPM.
dc.description.abstractMethods Eleven rats were studied under two different conditions: conscious or under inhalatory anaesthesia during F-18-FDG uptake. All animals were studied in vivo under both conditions in a dedicated small animal Philips MOSAIC PET scanner and magnetic resonance images were obtained for subsequent spatial processing. Then, rats were randomly assigned to a conscious or anaesthetized group for postmortem autoradiography, and slices from each animal were aligned and stacked to create a 3-D autoradiographic volume. Finally, differences in F-18-FDG uptake between conscious and anaesthetized states were assessed from PET and autoradiography data by SPM analysis and results were compared.
dc.description.abstractResults SPM results of PET and 3-D autoradiography are in good agreement and led to the detection of consistent cortical differences between the conscious and anaesthetized groups, particularly in the bilateral somatosensory cortices. However, SPM analysis of 3-D autoradiography also highlighted differences in the thalamus that were not detected with PET.
dc.description.abstractConclusion This study demonstrates that any difference detected with SPM analysis of MOSAIC PET images of rat brain is detected also by the gold standard autoradiographic technique, confirming that this methodology provides reliable results, although partial volume effects might make it difficult to detect slight differences in small regions.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00259-011-1905-y
dc.identifier.issn1619-7070
dc.identifier.urihttps://doi.org/10.1007/s00259-011-1905-y
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95313
dc.identifier.wosidWOS:000297732700015
dc.issue.numero12
dc.language.isoen
dc.pagina.final2237
dc.pagina.inicio2228
dc.revistaEuropean journal of nuclear medicine and molecular imaging
dc.rightsacceso restringido
dc.subjectRat brain
dc.subjectPositron emission tomography
dc.subjectThree-dimensional autoradiography
dc.subjectSPM
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleStatistical parametric maps of <SUP>18</SUP>F-FDG PET and 3-D autoradiography in the rat brain: a cross-validation study
dc.typeartículo
dc.volumen38
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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