Plasma and intracellular membrane inositol 1,4,5-trisphosphate receptors mediate the Ca<SUP>2+</SUP> increase associated with the ATP-induced increase in ciliary beat frequency

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dc.contributor.authorBarrera, NP
dc.contributor.authorMorales, B
dc.contributor.authorVillalón, M
dc.date.accessioned2025-01-21T01:07:36Z
dc.date.available2025-01-21T01:07:36Z
dc.date.issued2004
dc.description.abstractAn increase in intracellular free Ca2+ concentration ([Ca2+](i)) has been shown to be involved in the increase in ciliary beat frequency (CBF) in response to ATP; however, the signaling pathways associated with inositol 1,4,5-trisphosphate (IP3) receptor-dependent Ca2+ mobilization remain unresolved. Using radioimmunoassay techniques, we have demonstrated the appearance of two IP3 peaks occurring 10 and 60 s after ATP addition, which was strongly correlated with a release of intracellular Ca2+ from internal stores and an influx of extracellular Ca2+, respectively. In addition, ATP-dependent Ca2+ mobilization required protein kinase C (PKC) and Ca2+/ calmodulin-dependent protein kinase II activation. We found an increase in PKC activity in response to ATP, with a peak at 60 s after ATP addition. Xestospongin C, an IP3 receptor blocker, significantly diminished both the ATP-induced increase in CBF and the initial transient [Ca2+](i) component. ATP addition in the presence of xestospongin C or thapsigargin revealed that the Ca2+ influx is also dependent on IP3 receptor activation. Immunofluorescence and confocal microscopic studies showed the presence of IP3 receptor types 1 and 3 in cultured ciliated cells. Immunogold electron microscopy localized IP3 receptor type 3 to the nucleus, the endoplasmic reticulum, and, interestingly, the plasma membrane. In contrast, IP3 receptor type 1 was found exclusively in the nucleus and the endoplasmic reticulum. Our study demonstrates for the first time the presence of IP3 receptor type 3 in the plasma membrane in ciliated cells and leads us to postulate that the IP3 receptor can directly trigger Ca2+ influx in response to ATP.
dc.fuente.origenWOS
dc.identifier.doi10.1152/ajpcell.00343.2003
dc.identifier.eissn1522-1563
dc.identifier.issn0363-6143
dc.identifier.urihttps://doi.org/10.1152/ajpcell.00343.2003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96355
dc.identifier.wosidWOS:000223762000032
dc.issue.numero4
dc.language.isoen
dc.pagina.finalC1124
dc.pagina.inicioC1114
dc.revistaAmerican journal of physiology-cell physiology
dc.rightsacceso restringido
dc.subjecttransduction mechanisms
dc.subjectP2Y receptor
dc.subjectcalcium influx
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePlasma and intracellular membrane inositol 1,4,5-trisphosphate receptors mediate the Ca<SUP>2+</SUP> increase associated with the ATP-induced increase in ciliary beat frequency
dc.typeartículo
dc.volumen287
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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