Identification, characterization, and expression of lipoxygenase genes in sweet cherry (Prunus avium L.) cv. Regina and their relationship with the development of an herbaceous off-flavor during fruit ripening

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dc.contributor.authorVillavicencio, Juan David
dc.contributor.authorTobar, Jose
dc.contributor.authorZoffoli, Juan Pablo
dc.contributor.authorO'Brien, Jose Antonio
dc.contributor.authorContreras, Carolina
dc.date.accessioned2025-01-20T17:09:43Z
dc.date.available2025-01-20T17:09:43Z
dc.date.issued2024
dc.description.abstractFlavor is an essential characteristic of fruit quality and is significant for consumers. Off-flavors have been reported in several fruits, including sweet cherry. This fruit has been reported to show an herbaceous/grassy-like flavor. The herbaceous off-flavor in sweet cherries detected in cultivar Regina has been related to the differential development of aroma compounds such as short-chain aldehydes and esters. One of the main biosynthesis pathways for these compounds is the fatty acid oxidation mediated by lipoxygenases (LOX). In order to have a better understanding of the biological basis of the differences in the volatile profile, the LOX gene expression profile was characterized during fruit development with and without herbaceous off-flavor. A genome-wide analysis of LOX in sweet cherry was carried out and compared to other species such as Arabidopsis, tomato, apple, prunus and strawberry. The structural features of 9-LOX and 13-LOX genes, encoded protein domains and their synteny were examined. Moreover, we analyzed the LOX expression at four developmental stages along ripening by RT-qPCR. Thirteen LOX gene candidates (six 9-LOX and seven 13-LOX) were identified. The 13-LOXs, PaLOX10, PaLOX11, and PaLOX12 were differentially expressed in herbaceous sweet cherries. Furthermore, their expression profile positively correlated with key volatile compounds linked to the herbaceous off-flavor. Overall, this study involves the genome-wide characterization of the LOX family in Prunus avium cv. Regina and provides information that can aid in studying LOX-related fruit deterioration in sweet cherries and associated species.
dc.description.funderThe 'Fondo Nacional de Desarrollo Cientifico y Tecnologico' FONDECYT (Chile)
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.plaphy.2023.108271
dc.identifier.eissn1873-2690
dc.identifier.issn0981-9428
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2023.108271
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91066
dc.identifier.wosidWOS:001147266500001
dc.language.isoen
dc.revistaPlant physiology and biochemistry
dc.rightsacceso restringido
dc.subjectSweet cherry
dc.subjectLipoxygenase (LOX)
dc.subjectVolatiles
dc.subjectHerbaceous
dc.subjectOff-flavor
dc.titleIdentification, characterization, and expression of lipoxygenase genes in sweet cherry (Prunus avium L.) cv. Regina and their relationship with the development of an herbaceous off-flavor during fruit ripening
dc.typeartículo
dc.volumen206
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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