CARNITINE ACYLTRANSFERASE AND ACYL-COENZYME-A HYDROLASE ACTIVITIES IN HUMAN-LIVER - QUANTITATIVE-ANALYSIS OF THEIR SUBCELLULAR-LOCALIZATION

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dc.contributor.authorBRONFMAN, M
dc.contributor.authorLEIGHTON, F
dc.date.accessioned2025-01-23T19:43:44Z
dc.date.available2025-01-23T19:43:44Z
dc.date.issued1984
dc.description.abstractThe subcellular localizations of carnitine acyltransferase and acyl CoA hydrolase activities with different chain-length substrates were quantitatively evaluated in human liver by fractionation of total homogenates in metrizamide density gradients and by differential centrifugation. Peroxisomes contain 8-37% of the liver acyltransferase activity, the relative amount depending on the chain length of the substrate. The remaining activity was ascribed to mitochondria, except for carnitine octanoyltransferase, for which 25% of the activity was present in microsomal fractions. In contrast with rat liver, where the activity in peroxisomes is very low or absent, human liver peroxisomes contain about 20% of the carnitine palmitoyltransferase. Short-chain acyl CoA hydrolase activity was localized mainly in the mitochondrial and soluble compartments; the long-chain activity was present in both microsomal fractions and the soluble compartment. Particle-bound acyl CoA hydrolase activity for medium-chain substrates exhibited an intermediate distribution, in mictochondria and microsomal fractions, with 30-40% of the activity in the soluble fraction. No acyl CoA hydrolase activity appears to be present in human liver peroxisomes.
dc.fuente.origenWOS
dc.identifier.eissn1470-8728
dc.identifier.issn0264-6021
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99790
dc.identifier.wosidWOS:A1984TY10800005
dc.issue.numero3
dc.language.isoen
dc.pagina.final730
dc.pagina.inicio721
dc.revistaBiochemical journal
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCARNITINE ACYLTRANSFERASE AND ACYL-COENZYME-A HYDROLASE ACTIVITIES IN HUMAN-LIVER - QUANTITATIVE-ANALYSIS OF THEIR SUBCELLULAR-LOCALIZATION
dc.typeartículo
dc.volumen224
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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