DEOXYRIBONUCLEIC-ACID POLYMERASE FROM THE MARINE PSEUDOMONAS SP BAL-31

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dc.contributor.authorVICUNA, R
dc.contributor.authorVALDES, F
dc.contributor.authorMEDINA, A
dc.contributor.authorYUDELEVICH, A
dc.date.accessioned2025-01-23T19:44:31Z
dc.date.available2025-01-23T19:44:31Z
dc.date.issued1980
dc.description.abstractA DNA-dependent DNA polymerase (DNA nucleotidyltransferase) was purified 3000-fold from the marine Pseudomonas sp. BAL-31. The MW of the native enzyme was estimated by glycerol gradient sedimentation to be 110,000. The enzyme migrated in sodium dodecyl sulfate-acrylamide gels as a single polypeptide with a MW of 105,000. An absolute requirement for divalent cation was satisfied by Mg2+ or Mn2+ at concentrations of 1 mM. Monovalent cations at concentrations higher than 50 mM showed an inhibitory effect. The polymerase activity was resistant to N-ethylmaleimide and showed a wide pH optimum.
dc.fuente.origenWOS
dc.identifier.eissn1098-5530
dc.identifier.issn0021-9193
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/100066
dc.identifier.wosidWOS:A1980JQ95200032
dc.issue.numero1
dc.language.isoen
dc.pagina.final253
dc.pagina.inicio249
dc.revistaJournal of bacteriology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleDEOXYRIBONUCLEIC-ACID POLYMERASE FROM THE MARINE PSEUDOMONAS SP BAL-31
dc.typeartículo
dc.volumen142
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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