Nanomechanical and Molecular Characterization of Aging in Dentinal Collagen

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dc.contributor.authorSchuh, C.M.A.P.
dc.contributor.authorLeiva-Sabadini, C.
dc.contributor.authorHuang, S.
dc.contributor.authorBarrera, N.P.
dc.contributor.authorBozec, L.
dc.contributor.authorAguayo, S.
dc.date.accessioned2023-05-19T20:50:30Z
dc.date.available2023-05-19T20:50:30Z
dc.date.issued2022
dc.description.abstractMethylglyoxal (MGO) is an important molecule derived from glucose metabolism with the capacity of attaching to collagen and generating advanced glycation end products (AGEs), which accumulate in tissues over time and are associated with aging and diseases. However, the accumulation of MGO-derived AGEs in dentin and their effect on the nanomechanical properties of dentinal collagen remain unknown. Thus, the aim of the present study was to quantify MGO-based AGEs in the organic matrix of human dentin as a function of age and associate these changes with alterations in the nanomechanical and ultrastructural properties of dentinal collagen. For this, 12 healthy teeth from 50-y-old patients were collected and prepared to obtain crown and root dentin discs. Following demineralization, MGO-derived AGEs were quantified with a competitive ELISA. In addition, atomic force microscopy nanoindentation was utilized to measure changes in elastic modulus in peritubular and intertubular collagen fibrils. Finally, principal component analysis was carried out to determine aging profiles for crown and root dentin. Results showed an increased presence of MGO AGEs in the organic matrix of dentin in the >50-y-old specimens as compared with the <26-y-old specimens in crown and root. Furthermore, an increase in peritubular and intertubular collagen elasticity was observed in the >50-y-old group associated with ultrastructural changes in the organic matrix as determined by atomic force microscopy analysis. Furthermore, principal component analysis loading plots suggested different "aging profiles" in crown and root dentin, which could have important therapeutic implications in restorative and adhesive dentistry approaches. Overall, these results demonstrate that the organic matrix of human dentin undergoes aging-related changes due to MGO-derived AGEs with important changes in the nanomechanical behavior of collagen that may affect diagnostic and restorative procedures in older people.
dc.fechaingreso.objetodigital2024-12-07
dc.fechaingreso.objetodigitalNo aplica
dc.fuente.origenORCID-mayo23
dc.identifier.doi10.1177/00220345211072484
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/70279
dc.identifier.wosidWOS:000758914500001
dc.issue.numeroNo. 7
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final847
dc.pagina.inicio840
dc.revistaJournal of Dental Research
dc.rightsacceso restringido
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleNanomechanical and Molecular Characterization of Aging in Dentinal Collagenes_ES
dc.typeartículo
dc.volumenVol. 101
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