Free radical scavengers from <i>Cymbopogon citratus</i> (DC.) stapf plants cultivated in bioreactors by the temporary immersion (TIS) principle

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dc.contributor.authorTapia, Alejandro
dc.contributor.authorCheel, Jose
dc.contributor.authorTheoduloz, Cristina
dc.contributor.authorRodriguez, Jaime
dc.contributor.authorSchmeda-Hirschmann, Guillermo
dc.contributor.authorGerth, Andre
dc.contributor.authorWilken, Dirk
dc.contributor.authorJordan, Miguel
dc.contributor.authorJimenez-Gonzalez, Elio
dc.contributor.authorGomez-Kosky, Rafael
dc.contributor.authorQuiala Mendoza, Elisa
dc.date.accessioned2025-01-21T01:05:33Z
dc.date.available2025-01-21T01:05:33Z
dc.date.issued2007
dc.description.abstractThe biomass production of Cynibopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO2-enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect of the methanolic extracts was measured. From the TIS-culture of C citratus, seven compounds were isolated and identified as caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3), p-hydroxybenzoic acid (4), p-hydroxybenzoic acid 3-O-beta-D-glUcoside (5), glutamic acid (6) and luteolin 6-C-fucopyranoside (7). The occurrence of compounds 1-7 and their variability in C citratus grown under different TIS conditions was determined by HPLC. The free radical scavenging effect of the methanolic extract and compounds was measured by the discoloration of the free radical 1,1diphenyl-2-picrylhydrazyl (DPPH). The main metabolites in 6- and 8-week-old cultures, both in 5 and 10 1 vessels, were chlorogenic acid (2) (100-113 mg%) and neochlorogenic acid (3) (80119 mg%), while in the cultures with CO2-enriched air and reduced immersion frequency the main compound detected in the extracts was glutamic acid (6) (400 and 670 mg% for the green and white biomass and 619 and 630 mg% for the green and white biomass, respectively). The most active compounds, as free radical scavengers, in the DPPH discoloration assay were caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3) and the flavonoid luteolin 6-C-fucopyranoside (7).
dc.fuente.origenWOS
dc.identifier.eissn1865-7125
dc.identifier.issn0939-5075
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95954
dc.identifier.wosidWOS:000248400100021
dc.issue.numero5-6
dc.language.isoen
dc.pagina.final457
dc.pagina.inicio447
dc.revistaZeitschrift fur naturforschung section c-a journal of biosciences
dc.rightsacceso restringido
dc.subjectCymbopogon citratus
dc.subjectTIS culture
dc.subjectfree radical scavengers
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleFree radical scavengers from <i>Cymbopogon citratus</i> (DC.) stapf plants cultivated in bioreactors by the temporary immersion (TIS) principle
dc.typeartículo
dc.volumen62
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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