ON THE MECHANISMS OF CELL UNCOUPLING INDUCED BY A TUMOR PROMOTER PHORBOL ESTER IN CLONE-9 CELLS, A RAT-LIVER EPITHELIAL-CELL LINE

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dc.contributor.authorBERTHOUD, VM
dc.contributor.authorROOK, MB
dc.contributor.authorTRAUB, O
dc.contributor.authorHERTZBERG, EL
dc.contributor.authorSAEZ, JC
dc.date.accessioned2025-01-23T19:21:32Z
dc.date.available2025-01-23T19:21:32Z
dc.date.issued1993
dc.description.abstractIt is known that in Clone 9 (C9) cells, intercellular gap junctional communication (IGJC) is rapidly blocked by the tumor promoter phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), but it recovers spontaneously a few hours later and becomes refractory to TPA (Yada et al., J. Membr. Biol. 88, 217-232 (1985)). We now report that gap junctions between C9 cells contain at least two junctional proteins, connexin26 (Cx26) and connexin43 (Cx43), and that the TPA-induced changes in IGJC correlate temporally to changes in the state of phosphorylation of Cx43. The latter changes were prevented by inhibition of protein kinase C. Phosphoamino acid analysis and two-dimensional tryptic peptide maps of P-32-labeled Cx43 showed that during the TPA-induced phosphorylation at least two of the phosphorylated forms of Cx43 were differentially phosphorylated in seryl residues as compared to control. TPA induced a drastic reduction in junctional conductance as well as a redistribution of unitary gap junction channel event sizes seen in control cells. These changes were associated with retrieval of Cxs from the plasma membrane. Reappearance of gap junctions formed by Cx43 but not by Cx26 accounted for the spontaneous recovery in IGJC. It is proposed that gap junctions between C9 cells contain two types of channels each formed by Cx43 or Cx26 and that they are differentially affected during the action of TPA.
dc.fuente.origenWOS
dc.identifier.issn0171-9335
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98799
dc.identifier.wosidWOS:A1993MP84500020
dc.issue.numero2
dc.language.isoen
dc.pagina.final396
dc.pagina.inicio384
dc.revistaEuropean journal of cell biology
dc.rightsacceso restringido
dc.subjectINTERCELLULAR CHANNELS
dc.subjectTUMOR PROMOTOR
dc.subjectGAP JUNCTIONAL PROTEIN
dc.subjectPOSTTRANSLATIONAL MODIFICATION
dc.subjectUNITARY CONDUCTANCE
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleON THE MECHANISMS OF CELL UNCOUPLING INDUCED BY A TUMOR PROMOTER PHORBOL ESTER IN CLONE-9 CELLS, A RAT-LIVER EPITHELIAL-CELL LINE
dc.typeartículo
dc.volumen62
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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