Genome-Wide Characterization of Light-Regulated Gene Expression in<i> Botrytis cinerea</i> Reveals Underlying Complex Photobiology

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dc.contributor.authorPerez-Lara, Gabriel
dc.contributor.authorOlivares-Yanez, Consuelo
dc.contributor.authorvan Bakel, Harm
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.date.accessioned2025-01-20T20:11:30Z
dc.date.available2025-01-20T20:11:30Z
dc.date.issued2023
dc.description.abstractBotrytis cinerea is a necrotrophic fungus characterized mainly by its wide host range of infected plants. The deletion of the white-collar-1 gene (bcwcl1), which encodes for a blue-light receptor/transcription factor, causes a decrease in virulence, particularly when assays are conducted in the presence of light or photocycles. However, despite ample characterization, the extent of the light-modulated transcriptional responses regulated by BcWCL1 remains unknown. In this study, pathogen and pathogen:host RNA-seq analyses, conducted during non-infective in vitro plate growth and when infecting Arabidopsis thaliana leaves, respectively, informed on the global gene expression patterns after a 60 min light pulse on the wild-type B05.10 or increment bcwcl1 B. cinerea strains. The results revealed a complex fungal photobiology, where the mutant did not react to the light pulse during its interaction with the plant. Indeed, when infecting Arabidopsis, no photoreceptor-encoding genes were upregulated upon the light pulse in the increment bcwcl1 mutant. Differentially expressed genes (DEGs) in B. cinerea under non-infecting conditions were predominantly related to decreased energy production in response to the light pulse. In contrast, DEGs during infection significantly differ in the B05.10 strain and the? bcwcl1 mutant. Upon illumination at 24 h post-infection in planta, a decrease in the B. cinerea virulence-associated transcripts was observed. Accordingly, after a light pulse, biological functions associated with plant defense appear enriched among light-repressed genes in fungus-infected plants. Taken together, our results show the main transcriptomic differences between wild-type B. cinerea B05.10 and ? bcwcl1 after a 60 min light pulse when growing saprophytically on a Petri dish and necrotrophically over A. thaliana.
dc.fuente.origenWOS
dc.identifier.doi10.3390/ijms24108705
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttps://doi.org/10.3390/ijms24108705
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/92150
dc.identifier.wosidWOS:000998020700001
dc.issue.numero10
dc.language.isoen
dc.revistaInternational journal of molecular sciences
dc.rightsacceso restringido
dc.subjectBotrytis cinerea
dc.subjectlight receptor
dc.subjectphotoreceptor
dc.subjectphytopathogen
dc.subjecttranscriptomics
dc.subjectwhite collar
dc.titleGenome-Wide Characterization of Light-Regulated Gene Expression in<i> Botrytis cinerea</i> Reveals Underlying Complex Photobiology
dc.typeartículo
dc.volumen24
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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