Mechanisms of paracrine regulation by fetal membranes of human uterine quiescence

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dc.contributor.authorCarvajal, Jorge A.
dc.contributor.authorVidal, Rossana J.
dc.contributor.authorCuello, Mauricio A.
dc.contributor.authorPoblete, Jose A.
dc.contributor.authorWeiner, Carl P.
dc.date.accessioned2025-01-21T01:06:05Z
dc.date.available2025-01-21T01:06:05Z
dc.date.issued2006
dc.description.abstractOBJECTIVE: To test the hypothesis that fetal membranes (chorion or amnion) release one or more factors responsible for myometrial quiescence.
dc.description.abstractMETHODS: Myometrial samples were excised from women, at elective term cesarean delivery prior to the onset of labor. Fetal membranes were obtained after cesarean deliver), either before or during labor, and either term (greater than 37 weeks) or preterm (less than or equal to 36 weeks). Myometrial strips were placed in organ baths and contractions stimulated by oxytocin (10(-8) M). Contractility was measured under isometric conditions before and after exposure to fetal membranes or conditioned medium. The impact of either membrane or conditioned media on contractility was determined before and after myometrial K+ channel blockade.
dc.description.abstractRESULTS: Both chorion and amnion and their respective conditioned mediums decrease oxytocin-stimulated myometrial contraction. The inhibitory effect was greatest with membranes from preterm pregnancies (mean gestation 32 weeks, P <. 05). The inhibitory effect was detectable in the presence of term labor, but was absent when the fetal membranes were obtained after preterm labor. Iberiotoxin, an inhibitor of large conductance Ca2+-activated K+ channels (BKCa) reduced the effect of fetal membranes by 50% (P <. 05).
dc.description.abstractCONCLUSION: We conclude that human fetal membranes release one or more factors that inhibit oxytocin-induced myometrial contractility. We suggest this factor (or factors) acts mainly by opening myometrial BKCa. The findings further support our hypothesis that the fetal membranes release a factor (or factors) that is central to myometrial quiescence and its premature loss leads to preterm delivery.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.jsgi.2006.04.005
dc.identifier.issn1071-5576
dc.identifier.urihttps://doi.org/10.1016/j.jsgi.2006.04.005
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96084
dc.identifier.wosidWOS:000239044000004
dc.issue.numero5
dc.language.isoen
dc.pagina.final349
dc.pagina.inicio343
dc.revistaJournal of the society for gynecologic investigation
dc.rightsacceso restringido
dc.subjectmyometrial quiescence
dc.subjectfetal membranes
dc.subjectchorion
dc.subjectamnion
dc.subjectBKCa
dc.subject.ods05 Gender Equality
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa05 Igualdad de género
dc.subject.odspa03 Salud y bienestar
dc.titleMechanisms of paracrine regulation by fetal membranes of human uterine quiescence
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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