PEROXISOMAL FATTY-ACID OXIDATION AND INHIBITORS OF THE MITOCHONDRIAL CARNITINE PALMITOYLTRANSFERASE-I IN ISOLATED RAT HEPATOCYTES

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dc.contributor.authorSKORIN, C
dc.contributor.authorNECOCHEA, C
dc.contributor.authorJOHOW, V
dc.contributor.authorSOTO, U
dc.contributor.authorGRAU, AM
dc.contributor.authorBREMER, J
dc.contributor.authorLEIGHTON, F
dc.date.accessioned2025-01-23T19:22:22Z
dc.date.available2025-01-23T19:22:22Z
dc.date.issued1992
dc.description.abstractFatty acid oxidation was studied in the presence of inhibitors of carnitinic palmitoyltransferase I (CPT I), in normal and in peroxisome-proliferated rat hepatocytes. The oxidation decreased in mitochondria, as expected, but in peroxisomes it increased. These two effects were seen, in variable proportions, with (+)-decanoylcarnitine, 2-tetradecylglycidic acid (TDGA) and etomoxir. The decrease in mitochondrial oxidation (ketogenesis) affected saturated fatty acids with 12 or more carbon atoms, whereas the increase in peroxisomal oxidation (H2O2 production) affected saturated fatty acids with 8 or more carbon atoms. The peroxisomal increase was sensitive to chlorpromazine, a peroxisomal inhibitor. To study possible mechanisms, palmitoyl-, octanoyl- and acetyl-carnitine acyltransferase activities were measured, in homogenates and in subcellular fractions from control and TDGA-treated cells. The palmitoylcarnitine acyltransferase was inhibited, as expected, but the octanoyltransferase activity also decreased. The CoA derivative of TDGA was synthesized and tentatively identified as being responsible for inhibition of the octanoylcarnitine acyltransferase. These results show that inhibitors of the mitochondrial CPT 1 may also inhibit the peroxisomal octanoyl transferase; they also support the hypothesis that the octanoyltransferase has the capacity to control or regulate peroxisomal fatty acid oxidation.
dc.fuente.origenWOS
dc.identifier.eissn1470-8728
dc.identifier.issn0264-6021
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99021
dc.identifier.wosidWOS:A1992HB61500041
dc.language.isoen
dc.pagina.final567
dc.pagina.inicio561
dc.revistaBiochemical journal
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titlePEROXISOMAL FATTY-ACID OXIDATION AND INHIBITORS OF THE MITOCHONDRIAL CARNITINE PALMITOYLTRANSFERASE-I IN ISOLATED RAT HEPATOCYTES
dc.typeartículo
dc.volumen281
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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