HETEROLOGOUS EXPRESSION OF ESCHERICHIA-COLI PORIN GENES IN SALMONELLA-TYPHI TY2 - REGULATION BY MEDIUM OSMOLARITY, TEMPERATURE AND OXYGEN AVAILABILITY

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dc.contributor.authorCONTRERAS, I
dc.contributor.authorMUNOZ, L
dc.contributor.authorTORO, CS
dc.contributor.authorMORA, GC
dc.date.accessioned2025-01-21T01:34:25Z
dc.date.available2025-01-21T01:34:25Z
dc.date.issued1995
dc.description.abstractElectrophoretic analysis of outer membrane proteins showed that Salmonella typhi OmpC expression is not reciprocally regulated relative to OmpF as described for Escherichia coli and S. typhimurium. When bacteria were grown in minimal media, both OmpC and OmpF were repressed as the osmolarity increased. However, in Luria broth, expression of OmpC was slightly induced by osmolarity up to 0.3 osmM, Plasmids bearing E. coli ompC-lacZ or ompF-lacZ gene fusions were studied for their expression in S. typhi and E. coli. Under anaerobic growth conditions, expression of ompC-lacZ in S. typhi was maximal at 0.16 osmM, while in E. coli expression was maximal at 0.7 osmM. ompF-lacZ expression was similarly repressed by medium osmolarity and anaerobiosis in both species. In contrast, a drastic difference in the regulation of OmpF by temperature was observed; at 37 degrees C ompF-lacZ expression was repressed in E. coli, while in S. typhi it was induced.
dc.fuente.origenWOS
dc.identifier.eissn1574-6968
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97555
dc.identifier.wosidWOS:A1995TE09800018
dc.issue.numero1-2
dc.language.isoen
dc.pagina.final111
dc.pagina.inicio105
dc.revistaFems microbiology letters
dc.rightsacceso restringido
dc.subjectSALMONELLA TYPHI
dc.subjectOMPC
dc.subjectOMPF
dc.subjectREGULATION
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHETEROLOGOUS EXPRESSION OF ESCHERICHIA-COLI PORIN GENES IN SALMONELLA-TYPHI TY2 - REGULATION BY MEDIUM OSMOLARITY, TEMPERATURE AND OXYGEN AVAILABILITY
dc.typeartículo
dc.volumen133
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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