TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity

Simple item page
dc.contributor.authorAndrade, YN
dc.contributor.authorFernandes, J
dc.contributor.authorVázquez, E
dc.contributor.authorFernández-Fernández, JM
dc.contributor.authorArniges, M
dc.contributor.authorSánchez, TM
dc.contributor.authorVillalón, M
dc.contributor.authorValverde, MA
dc.date.accessioned2025-01-21T01:07:04Z
dc.date.available2025-01-21T01:07:04Z
dc.date.issued2005
dc.description.abstractAutoregulation of the ciliary beat frequency (CBF) has been proposed as the mechanism used by,A epithelial ciliated cells to maintain the CBF and prevent the collapse of mucociliary transport under conditions of varying mucus viscosity. Despite the relevance of this regulatory response to the pathophysiology of airways and reproductive tract, the underlying cellular and molecular aspects remain unknown. Hamster oviductal ciliated cells express the transient receptor potential vanilloid 4 (TRPV4) channel, which is activated by increased viscous load involving a phospholipase A(2)-dependent pathway. TRPV4-transfected HeLa cells also increased their cationic currents in response to high viscous load. This mechanical activation is prevented in native ciliated cells loaded with a TRPV4 antibody. Application of the TRPV4 synthetic ligand 4 alpha-phorbol 12,13-didecanoate increased cationic currents, intracellular Ca2+, and the CBF in the absence of a viscous load. Therefore, TRPV4 emerges as a candidate to participate in the coupling of fluid viscosity changes to the generation of the Ca2+ signal required for the autoregulation of CBF.
dc.fuente.origenWOS
dc.identifier.issn0021-9525
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96272
dc.identifier.wosidWOS:000227843200005
dc.issue.numero6
dc.language.isoen
dc.pagina.final874
dc.pagina.inicio869
dc.revistaJournal of cell biology
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity
dc.typeartículo
dc.volumen168
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas