Heterologous expression of Escherichia coli ppsA (phosphoenolpyruvate synthetase) and galU (UDP-glucose pyrophosphorylase) genes in Corynebacterium glutamicum, and its impact on trehalose synthesis

Simple item page
dc.contributor.authorPadilla, L
dc.contributor.authorAgosin, E
dc.date.accessioned2024-01-10T12:05:48Z
dc.date.available2024-01-10T12:05:48Z
dc.date.issued2005
dc.description.abstractTrehalose is a disaccharide with a wide range of applications in the food industry. We recently proposed a strategy for trehalose production based on a Corynebacterium glutamicum strain expressing the Escherichia coli enzyme UDP-glucose pyrophosphorylase (GalU).
dc.description.abstractBiochemical network analysis suggest a further bottleneck for trehalose synthesis resulting from the coupling of phosphotransferase (PTS) mediated glucose uptake, and glucose catabolism in C glutamicum. To overcome this coupling, we propose the expression of E coli phosphoenolpyruvate synthetase (PpsA), in addition to GalU expression, in C glutamicum.
dc.description.abstractAlthough GalU expression improved trehalose synthesis in C glutamicum, the simultaneous expression of GalU and PpsA did not result in a further increase in trehalose yield, but resulted in an increased catabolic rate of glucose, which could be ascribed to the operation of a futile cycle between phosphoenolpyruvate and pyruvate.
dc.description.abstractThe impact of GalU and PpsA expression on polysaccharide content, side product excretion and metabolic fluxes is discussed, as well as alternative ways to decouple glucose uptake and catabolism, in order to increase trehalose yield. (c) 2005 Elsevier Inc. All rights reserved.
dc.fechaingreso.objetodigital16-04-2024
dc.format.extent9 páginas
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.ymben.2005.04.001
dc.identifier.eissn1096-7184
dc.identifier.issn1096-7176
dc.identifier.pubmedidMEDLINE:15949962
dc.identifier.urihttps://doi.org/10.1016/j.ymben.2005.04.001
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/76071
dc.identifier.wosidWOS:000232079600003
dc.information.autorucIngeniería;Agosin E;S/I;99630
dc.issue.numero4
dc.language.isoen
dc.nota.accesoContenido parcial
dc.pagina.final268
dc.pagina.inicio260
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.revistaMETABOLIC ENGINEERING
dc.rightsacceso restringido
dc.subjectCorynebacterium glutamicum
dc.subjectPpsA
dc.subjectGalU
dc.subjecttrehalose
dc.subjectMETABOLIC CAPABILITIES
dc.subjectGLYCOGEN-SYNTHESIS
dc.subjectTRANSFORMATION
dc.subjectSYNTHASE
dc.subjectENZYME
dc.subjectDNA
dc.subject.ods12 Responsible Consumption and Production
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa12 Producción y consumo responsable
dc.subject.odspa03 Salud y bienestar
dc.titleHeterologous expression of Escherichia coli ppsA (phosphoenolpyruvate synthetase) and galU (UDP-glucose pyrophosphorylase) genes in Corynebacterium glutamicum, and its impact on trehalose synthesis
dc.typeartículo
dc.volumen7
sipa.codpersvinculados99630
sipa.indexWOS
sipa.trazabilidadCarga SIPA;09-01-2024
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2024-04-16. Heterologous expression of Escherichia coli ppsA (phosphoenolpyruvate synthetase) and galU (UDP-glucose pyrophosphorylase) genes in Corynebacterium glutamicum.pdf
Size:
2.84 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Artículos de revistas