Effect of manganese on the secretion of manganese-peroxidase by the basidiomycete <i>Ceriporiopsis subvermispora</i>

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dc.contributor.authorMancilla, Rodrigo A.
dc.contributor.authorCanessa, Paulo
dc.contributor.authorManubens, Augusto
dc.contributor.authorVicuna, Rafael
dc.date.accessioned2025-01-21T00:05:34Z
dc.date.available2025-01-21T00:05:34Z
dc.date.issued2010
dc.description.abstractThe ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic activity assays or Western blot hybridizations. In the corresponding mycelia, immuno-electron microscopy experiments showed high levels of MnP enzyme within intracellular compartments. These results suggest that in addition to its well-known effect on transcription regulation of mnp genes, manganese influences secretion of MnP to the extracellular medium. Experiments carried out in the presence of cycloheximide confirmed that the metal is required to secrete MnP already synthesized and retained within the cell. (C) 2010 Elsevier Inc. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.fgb.2010.04.003
dc.identifier.eissn1096-0937
dc.identifier.issn1087-1845
dc.identifier.urihttps://doi.org/10.1016/j.fgb.2010.04.003
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95553
dc.identifier.wosidWOS:000278855300009
dc.issue.numero7
dc.language.isoen
dc.pagina.final661
dc.pagina.inicio656
dc.revistaFungal genetics and biology
dc.rightsacceso restringido
dc.subjectLigninolysis
dc.subjectMnP
dc.subjectProtein secretion
dc.subjectManganese
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleEffect of manganese on the secretion of manganese-peroxidase by the basidiomycete <i>Ceriporiopsis subvermispora</i>
dc.typeartículo
dc.volumen47
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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