Structure and expression of a laccase gene from the ligninolytic basidiomycete Ceriporiopsis subvermispora

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dc.contributor.authorKarahanian, E
dc.contributor.authorCorsini, G
dc.contributor.authorLobos, S
dc.contributor.authorVicuña, R
dc.date.accessioned2025-01-21T01:32:25Z
dc.date.available2025-01-21T01:32:25Z
dc.date.issued1998
dc.description.abstractA gene encoding laccase has been isolated from a genomic library of the white-rot basidiomycete Ceriporiopsis subvermispora constructed in Lambda GEM-ii. This gene (Cs-lcs1) contains an open reading frame of 2215 bp, encoding a mature protein of 499 amino acids with a 21-residue signal peptide. The protein sequence exhibits between 63 and 68% identity with laccases from other basidiomycetes and shares with all of them 10 conserved histidines and one cysteine involved in the coordination of copper atoms at the active site of the enzyme. The gene possesses ii introns, with splicing junctions and internal lariat formation sites adhering to the GT-AG and CTRAY rules, respectively. The upstream region of Cs-lcsl contains a TATA box, two CAAT sites, five putative metal response elements and a ACE1 element. In agreement with the presence of the latter element, transcription of Cs-lcsl is activated by copper and silver, as shown by Northern blot and reverse transcription followed by DNA amplification analyses. Based on Southern blot analysis, Cs-lcsl appears to be the only gene encoding laccase in C. subvermispora. (C) 1998 Elsevier Science B.V. All rights reserved.
dc.description.urihttps://doi.org/10.1016/S0167-4781(98)00197-3
dc.fechaingreso.objetodigital2025-04-01
dc.fuente.origenWOS
dc.identifier.issn0167-4781
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97258
dc.identifier.wosidWOS:000077781400005
dc.issue.numeroNo. 1-2
dc.language.isoen
dc.nota.accesocontenido parcial
dc.pagina.final74
dc.pagina.inicio65
dc.revistaBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression
dc.rightsacceso restringido
dc.subjectlignin biodegradation
dc.subjectwhite-rot fungus
dc.subjectphenol oxidase
dc.subjectgenomic clone
dc.subjectACE1
dc.subjectmetal response element
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleStructure and expression of a laccase gene from the ligninolytic basidiomycete Ceriporiopsis subvermispora
dc.typeartículo
dc.volumenVol. 1443
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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