Characterization and Pathogenicity of <i>Diplodia</i>, <i>Lasiodiplodia</i>, and <i>Neofusicoccum</i> Species Causing Botryosphaeria Canker and Dieback of Apple Trees in Central Chile

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dc.contributor.authorDiaz, Gonzalo A.
dc.contributor.authorValdez, Adrian
dc.contributor.authorHalleen, Francois
dc.contributor.authorFerrada, Enrique
dc.contributor.authorLolas, Mauricio
dc.contributor.authorLatorre, Bernardo A.
dc.date.accessioned2025-01-20T21:04:35Z
dc.date.available2025-01-20T21:04:35Z
dc.date.issued2022
dc.description.abstractIn recent years, the number of apple trees affected by Botryosphaeria cankers and dieback has considerably increased in central Chile. This study aimed to identify the species of Botryosphaeriaceae associated with canker and dieback symptoms, estimate disease incidence and distributions, and study their pathogenicity and virulence on apple and other fruit crops. A field survey of 34 commercial orchards of apple (7 to 30 years old) was conducted in 16 localities, obtaining 270 symptomatic branch and trunk samples in 2017 and 2018 growing seasons. The incidence of Botryosphaeria canker and dieback ranged between 5 and 40%, and a total of 255 isolates of Botryosphaeriaceae spp. were obtained from 238 cankers. Morphological identification along with phylogenetic studies of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rDNA, part of the translation elongation factor 1-alpha (tef1-alpha), and part of the beta-tubulin (tub2) genes allowed us to identify Diplodia mutila (n = 49 isolates), D. seriata (n = 136 isolates), Lasiodiplodia theobromae (n = 16 isolates), and Neofusicoccum arbuti (n = 54 isolates). L. theobromae was isolated mainly from apple dieback from northern localities. All pathogens tested were pathogenic, causing canker and dieback symptoms on lignified twigs of apple, pear, walnut, and green grapevine shoots in the field. Isolates of N. arbuti were the most virulent, reproducing more severe cankers on the lignified tissues inoculated. This study reports, for the first time, D. mutila and L. theobromae associated with Botryosphaeria canker and dieback in Chile, and it is the first description of N. arbuti causing apple dieback worldwide.
dc.fuente.origenWOS
dc.identifier.doi10.1094/PDIS-06-21-1291-RE
dc.identifier.eissn1943-7692
dc.identifier.issn0191-2917
dc.identifier.urihttps://doi.org/10.1094/PDIS-06-21-1291-RE
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/93249
dc.identifier.wosidWOS:000823088100016
dc.issue.numero3
dc.language.isoen
dc.pagina.final937
dc.pagina.inicio925
dc.revistaPlant disease
dc.rightsacceso restringido
dc.subjectapple dieback
dc.subjectDiplodia mutila
dc.subjectDiplodia seriata
dc.subjectLasiodiplodia theobromae
dc.subjectNeofusicoccum arbuti
dc.titleCharacterization and Pathogenicity of <i>Diplodia</i>, <i>Lasiodiplodia</i>, and <i>Neofusicoccum</i> Species Causing Botryosphaeria Canker and Dieback of Apple Trees in Central Chile
dc.typeartículo
dc.volumen106
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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