Comparative study of intracisternal kaolin injection techniques to induce congenital hydrocephalus in fetal lamb

dc.contributor.authorDuru, Soner
dc.contributor.authorOria, Marc
dc.contributor.authorArevalo, Silvia
dc.contributor.authorRodo, Carlota
dc.contributor.authorCorrea, Laura
dc.contributor.authorVuletin, Fernando
dc.contributor.authorSanchez-Margallo, Francisco
dc.contributor.authorPeiro, Jose L.
dc.date.accessioned2025-01-23T21:16:31Z
dc.date.available2025-01-23T21:16:31Z
dc.date.issued2019
dc.description.abstractPurpose Kaolin (aluminum silicate) has been used to generate hydrocephalus by direct cisterna magna injection in animal models. The aim of the present study is to compare which method of Kaolin injection into fetal cisterna magna is feasible, safer, and more effective to induce hydrocephalus in fetal lambs.
dc.description.abstractMethods Twenty-five well-dated pregnant ewes at gestational 85-90 days (E85-90) were used to compare three different kaolin injection puncture techniques into the fetal cisterna magna. Group 1, ultrasound guidance in a maternal percutaneous transabdominal (TA); group 2, without opening the uterus in a transuterine (TU) technique; group 3, by occipital direct access after exteriorizing fetal head (EFH); and group 4, control group, was normal fetal lambs without injection. The fetal lambs were assessed using lateral ventricle diameter ultrasonographic measurements prior the kaolin injection and on the subsequent days. We analyzed the effectivity, mortality, and fetal losses to determine the best technique to create hydrocephalus in fetal lamb.
dc.description.abstractResults After fetal intracisternal kaolin (2%, 1mL) injection, lateral ventricle diameters increased progressively in the three different interventional groups compared with the normal values of the control group (p <= 0.05). We observed that the transabdominal method had a 60% of fetal losses, considering failure of injection and mortality, compared with the 12.5% in the open group (EFH), and 0% for the transuterine group.
dc.description.abstractConclusions Based on our study, we believe that both, open uterine (EFH) and transuterine approaches are more effective and safer than the transabdominal ultrasound-guided method to induce hydrocephalus.
dc.description.funderProf. Jose L Peiro Internal Cincinnati Children's Hospital funding
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00381-019-04096-1
dc.identifier.eissn1433-0350
dc.identifier.issn0256-7040
dc.identifier.urihttps://doi.org/10.1007/s00381-019-04096-1
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/101124
dc.identifier.wosidWOS:000464882300016
dc.issue.numero5
dc.language.isoen
dc.pagina.final849
dc.pagina.inicio843
dc.revistaChilds nervous system
dc.rightsacceso restringido
dc.subjectFetal
dc.subjectHydrocephalus
dc.subjectKaolin
dc.subjectSheep
dc.subjectVentriculomegaly
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleComparative study of intracisternal kaolin injection techniques to induce congenital hydrocephalus in fetal lamb
dc.typeartículo
dc.volumen35
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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