Cloning and functional characterization of the gene encoding the transcription factor Ace1 in the basidiomycete <i>Phanerochaete chrysosporium</i>

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dc.contributor.authorPolanco, Ruben
dc.contributor.authorCanessa, Paulo
dc.contributor.authorRivas, Alexis
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorLobos, Sergio
dc.contributor.authorVicuna, Rafael
dc.date.accessioned2025-01-21T01:06:27Z
dc.date.available2025-01-21T01:06:27Z
dc.date.issued2006
dc.description.abstractIn this report we describe the isolation and characterization of a gene encoding the transcription factor Ace 1 (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-ace 1 encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Ace 1 Mac 1 and Haa 1 from Saccharomyces cerevisiae. The Pc-ace 1 gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae ace 1 null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-ace 1. Moreover, Northern blot hybridization studies indicated that Pc-ace 1 cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Ace 1 transcription factor in basidiomycetes.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96160
dc.identifier.wosidWOS:000248288400007
dc.issue.numero4
dc.language.isoen
dc.pagina.final648
dc.pagina.inicio641
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectAce1
dc.subjectbasidiomycete
dc.subjectcopper
dc.subjectPhanerochaete chrysosporium
dc.subject.ods06 Clean Water and Sanitation
dc.subject.odspa06 Agua limpia y saneamiento
dc.titleCloning and functional characterization of the gene encoding the transcription factor Ace1 in the basidiomycete <i>Phanerochaete chrysosporium</i>
dc.typeartículo
dc.volumen39
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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