Histamine reduces gap junctional communication of human tonsil high endothelial cells in culture

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dc.contributor.authorFigueroa, XF
dc.contributor.authorAlviña, K
dc.contributor.authorMartínez, AD
dc.contributor.authorGarcés, G
dc.contributor.authorRosemblatt, M
dc.contributor.authorBoric, MP
dc.contributor.authorSáez, JC
dc.date.accessioned2025-01-21T01:07:31Z
dc.date.available2025-01-21T01:07:31Z
dc.date.issued2004
dc.description.abstractThe regulation of gap junctional communication by histamine was studied in primary cultures of human tonsil high endothelial cells (HUTECs). We evaluated intercellular communication, levels, state of phosphorylation, and cellular distribution of gap junction protein subunits, mainly connexin (Cx)43. Histamine induced a time-dependent reduction in dye coupling (Lucifer yellow) associated with reduction in connexin43 localized at cell-cell appositions (immunofluorescence), without changes in levels and phosphorylation state of connexin43 (immunoblots). These effects were prevented with chlorpheniramine, an H-1 receptor blocker; indomethacin, a cyclooxygenase blocker; or GF109203X, a protein kinase C inhibitor. Treatment with phorbol myristate acetate, a protein kinase C activator, and 4bromo (4Br)-A23187, a calcium ionophore, mimicked the histamine-induced effects on dye coupling. 8Bromo-cAMP doubled the dye coupling extent and prevented the histamine-induced reduction in incidence of dye coupling. After 24-h histamine treatment, known to desensitize H, receptors, reapplication of histamine increased cell coupling in a way prevented by ranitidine, an H-2 receptor blocker. Thus, activation of H-1 and H-2 receptors, which increase intracellular levels of free Ca2+ and cAMP, respectively, may affect gap junctional communication in opposite ways. Stabilization of actin filaments with phalloidine diminished but did not totally prevent histamine-induced cell shape changes and reduction in dye coupling. Hence, the histamine-induced reduction in gap junctional communication between HUTEC is mediated by cytoskeleton-dependent and -independent mechanisms and might contribute to modulate endothelial function in lymphoid tissue. (C) 2004 Elsevier Inc. All fights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.mvr.2004.06.009
dc.identifier.eissn1095-9319
dc.identifier.issn0026-2862
dc.identifier.urihttps://doi.org/10.1016/j.mvr.2004.06.009
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96340
dc.identifier.wosidWOS:000224910000012
dc.issue.numero3
dc.language.isoen
dc.pagina.final257
dc.pagina.inicio247
dc.revistaMicrovascular research
dc.rightsacceso restringido
dc.subjectConnexin43
dc.subjectdye coupling
dc.subjectendothelium
dc.subjectcytoskeleton
dc.subjectgap junctions
dc.subjectinflammation
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHistamine reduces gap junctional communication of human tonsil high endothelial cells in culture
dc.typeartículo
dc.volumen68
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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