Corticotropin-releasing factor binding protein enters the regulated secretory pathway in neuroendocrine cells and cortical neurons

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dc.contributor.authorBlanco, Elias H.
dc.contributor.authorPablo Zuniga, Juan
dc.contributor.authorEstela Andres, Maria
dc.contributor.authorAlvarez, Alejandra R.
dc.contributor.authorGysling, Katia
dc.date.accessioned2025-01-21T00:01:10Z
dc.date.available2025-01-21T00:01:10Z
dc.date.issued2011
dc.description.abstractCorticotropin releasing factor binding protein (CRF-BP) is a 37 kDa glycoprotein that binds CRF with high affinity. CRF-BP controls CRF levels within plasma during human pregnancy. It has also been shown that CRF-BP is expressed in various brain nuclei. Main actions that have been proposed for brain CRF-BP are either decreasing available CRF or facilitating CRF ligand-induced activation of CRF-R2 receptors. For both actions, it is necessary the release of CRF-BP from CRF-BP expressing neurons. However, the secretion mode of CRF-BP is currently unknown. We used heterologous expression of CRF-BP-Flag in PC12 cells and in primary culture of rat cortical neurons to study CRF-BP secretion mode. We observed that CRF-BP-Flag immunoreactivity presents the typical cytoplasmatic punctuate pattern that has been described for neuropeptides and proteins that enter the regulated secretory pathway in PC12 cells. Quantitative analysis of double immunofluorescence confocal images showed that CRF-BP-Flag colocalizes with secretogranin II, marker of secretory granules, both in PC12 and in primary-cultured rat neurons. Furthermore, CRF-BP-Flag is released from PC12 cells upon high K(+)-depolarization. Thus, our results show that CRF-BP is efficiently sorted to the regulated secretory pathway in two cellular contexts, suggesting that the extracellular levels of CRF-BP in the central nervous system depends on neuronal activity. (C) 2011 Elsevier Ltd. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.npep.2011.05.002
dc.identifier.issn0143-4179
dc.identifier.urihttps://doi.org/10.1016/j.npep.2011.05.002
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95376
dc.identifier.wosidWOS:000295349600003
dc.issue.numero4
dc.language.isoen
dc.pagina.final279
dc.pagina.inicio273
dc.revistaNeuropeptides
dc.rightsacceso restringido
dc.subjectCorticotropin releasing hormone (CRH)
dc.subjectRelease
dc.subjectSorting
dc.subjectCRH-BP
dc.subjectBinding protein
dc.subjectNeuropeptides
dc.subjectRegulated secretory pathway
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleCorticotropin-releasing factor binding protein enters the regulated secretory pathway in neuroendocrine cells and cortical neurons
dc.typeartículo
dc.volumen45
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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