β-glucosidase from the grape native yeast <i>Debaryomyces vanrijiae</i>
| dc.contributor.author | Belancic, A | |
| dc.contributor.author | Gunata, Z | |
| dc.contributor.author | Vallier, MJ | |
| dc.contributor.author | Agosin, E | |
| dc.date.accessioned | 2025-01-21T01:09:42Z | |
| dc.date.available | 2025-01-21T01:09:42Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | Six hundred ten yeast colonies isolated from various vineyards in Chile were screened for the presence of a beta-glucosidase activity as well as the resistance to glucose and ethanol inhibition. Among them, Debaryomyces vanrijiae was found to produce high levels of an extracelular beta-glucosidase which was tolerant to glucose (K-i = 439 mM) and ethanol inhibitions. The enzyme (designated DV-BG) was purified to apparent homogeneity,respectively, by gel filtration, ion-exchange, and chromato-focusing techniques. Its molecular weight was 100 000, and its pl 3.0, optimum pH, and temperature activities were 5.0 and 40 degreesC, respectively, and had a V-max of 47.6 mumol min(-1) mg(-1) and a K-m of 1.07 mM. The enzyme was active against different beta-D-glucosides including glucosidic flavor precursors. The disaccharidic flavor precursors were not substrates for the enzyme. When added to a Muscat grape juice, the concentration of several monoterpenes increased as the consequence of its hydrolytic activity. | |
| dc.fuente.origen | WOS | |
| dc.identifier.doi | 10.1021/jf025777l | |
| dc.identifier.eissn | 1520-5118 | |
| dc.identifier.issn | 0021-8561 | |
| dc.identifier.uri | https://doi.org/10.1021/jf025777l | |
| dc.identifier.uri | https://repositorio.uc.cl/handle/11534/96610 | |
| dc.identifier.wosid | WOS:000181087100056 | |
| dc.issue.numero | 5 | |
| dc.language.iso | en | |
| dc.pagina.final | 1459 | |
| dc.pagina.inicio | 1453 | |
| dc.revista | Journal of agricultural and food chemistry | |
| dc.rights | acceso restringido | |
| dc.subject | beta-glucosidase | |
| dc.subject | Debaryomyces vanrijiae | |
| dc.subject | glucose inhibition | |
| dc.subject | aroma enhancement | |
| dc.title | β-glucosidase from the grape native yeast <i>Debaryomyces vanrijiae</i> | |
| dc.type | artículo | |
| dc.volumen | 51 | |
| sipa.index | WOS | |
| sipa.trazabilidad | WOS;2025-01-12 |