HYDROGEN-PEROXIDE IS INVOLVED IN HAMSTER SPERM CAPACITATION INVITRO

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dc.contributor.authorBIZE, I
dc.contributor.authorSANTANDER, G
dc.contributor.authorCABELLO, P
dc.contributor.authorDRISCOLL, D
dc.contributor.authorSHARPE, C
dc.date.accessioned2025-01-23T19:22:45Z
dc.date.available2025-01-23T19:22:45Z
dc.date.issued1991
dc.description.abstractWe have investigated the possibility that the generation of hydrogen peroxide (H2O2) by spermatozoa plays a physiological role during capacitation. Capacitation is defined as the incubation period required for fertilization in mammals. Capacitation culminates in an exocytotic event, the acrosome reaction (AR). Mammalian sperm generate H2O2 during aerobic incubation and do not contain catalase, the enzyme that promotes scavenging of H2O2. In the present work we show that added catalase inhibited the AR, while glucose oxidase (GO), an enzyme that generates H2O2, accelerated the onset of the AR. Direct addition of H2O2 also stimulated the AR; catalase inhibited both the stimulation by GO and by H2O2. The onset of the AR was always preceded by the appearance of hyperactivated motility. The stimulation of the AR by H2O2 was manifest 1-2 h after the addition of H2O2. Catalase added at 3 h of incubation was less effective in inhibiting the AR than catalase added at the beginning. Incubation of sperm with catalase prevented the induction of the AR by the membrane-perturbing lipid, lysophosphatidyl choline. Taken together, these results suggest that H2O2 produced by hamster sperm plays a significant role during capacitation, possibly in membrane reorganization to facilitate the fusion that takes place during exocytosis of the acrosomal contents.
dc.description.funderNCRR NIH HHS
dc.fuente.origenWOS
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99095
dc.identifier.wosidWOS:A1991EX84200002
dc.issue.numero3
dc.language.isoen
dc.pagina.final403
dc.pagina.inicio398
dc.revistaBiology of reproduction
dc.rightsacceso restringido
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHYDROGEN-PEROXIDE IS INVOLVED IN HAMSTER SPERM CAPACITATION INVITRO
dc.typeartículo
dc.volumen44
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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