Serological response to <i>Helicobacter pylori</i> recombinant antigens in Chilean infected patients with duodenal ulcer, non-ulcer dyspepsia and gastric cancer

Simple item page
dc.contributor.authorOpazo, P
dc.contributor.authorMüller, I
dc.contributor.authorRollán, A
dc.contributor.authorValenzuela, P
dc.contributor.authorYudelevich, A
dc.contributor.authorGarcía-de la Guarda, R
dc.contributor.authorUrra, S
dc.contributor.authorVenegas, A
dc.date.accessioned2025-01-21T01:31:47Z
dc.date.available2025-01-21T01:31:47Z
dc.date.issued1999
dc.description.abstractWe have previously cloned 10 Helicobacter pylori antigen genes from a Chilean strain including: cytotoxin VacA, a truncated region of CagA (called A17), a species-specific protein (Ag26), urease subunits (UreA, UreB), a flagellin, (FlaB), heat shock proteins (HspA and HspB), an adhesin (HpaA) and a lipoprotein (Lpp20). Immunogenicity of these antigens was tested by immunoblot with sera of Chilean infected patients, revealing that HpaA, A17, HspB and VacA were more frequently recognized (86%, 820/o, 68% and 68%, respectively). According to the clinical condition, it was determined that Lpp20 was preferentially recognized by sera from non-ulcer dyspepsia patients (80%), A17 and VacA by patients with duodenal ulcer (92% and 83% respectively), and HspB by patients with duodenal ulcer (83%) and gastric cancer (90%). An ELISA was developed with a purified mixture of A17 and VacA antigens to test the different groups of patients. It was found that sera from duodenal ulcer patients showed higher values than those from non-ulcer dyspepsia patients, but this difference was not significant (p<0.2). Moreover, sera from gastric cancer patients showed values lower than those from non-ulcer dyspepsia patients (p<0.019). These results indicate that, in the Chilean population, antibodies raised against VacA and A17 are not markers either for duodenal ulcer or for gastric cancer.
dc.fuente.origenWOS
dc.identifier.eissn1600-0463
dc.identifier.issn0903-4641
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/97141
dc.identifier.wosidWOS:000084375100003
dc.issue.numero12
dc.language.isoen
dc.pagina.final1078
dc.pagina.inicio1069
dc.revistaApmis
dc.rightsacceso restringido
dc.subjectHelicobacter pylori
dc.subjectCagA
dc.subjectVacA
dc.subjectH-pylori-cloned antigens
dc.subjectsera antibodies
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleSerological response to <i>Helicobacter pylori</i> recombinant antigens in Chilean infected patients with duodenal ulcer, non-ulcer dyspepsia and gastric cancer
dc.typeartículo
dc.volumen107
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas