Molecular characterization of resistance mechanisms to chloramphenicol in Shigella flexneri strains isolated from Chilean children with acute diarrhea

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dc.contributor.authorFarfán, M
dc.contributor.authorFlores, O
dc.contributor.authorNavarro, N
dc.contributor.authorPrado, V
dc.contributor.authorMora, G
dc.contributor.authorToro, C
dc.date.accessioned2025-01-21T01:30:28Z
dc.date.available2025-01-21T01:30:28Z
dc.date.issued2002
dc.description.abstractBackground: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chlorampbenicol (Cm-R) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the Cm-R phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chlorampbenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All Cm-R strains had a minimal inhibitory concentration over 64 mug/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chlorampbenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene.
dc.fuente.origenWOS
dc.identifier.eissn0717-6163
dc.identifier.issn0034-9887
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96774
dc.identifier.wosidWOS:000175413900005
dc.issue.numero3
dc.language.isoen
dc.pagina.final280
dc.pagina.inicio275
dc.revistaRevista medica de chile
dc.rightsacceso restringido
dc.subjectchloramphenical o-acetyl-transferase
dc.subjectchloramphenical resistance
dc.subjectgenes
dc.subjectcat
dc.subjectShigella flexneri
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMolecular characterization of resistance mechanisms to chloramphenicol in Shigella flexneri strains isolated from Chilean children with acute diarrhea
dc.typeartículo
dc.volumen130
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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