Functional analysis of the endoxylanase B (<i>xynB</i>) promoter from <i>Penicillium purpurogenum</i>

Simple item page
dc.contributor.authorDiaz, Jheimmy
dc.contributor.authorChavez, Renato
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorEyzaguirre, Jaime
dc.contributor.authorBull, Paulina
dc.date.accessioned2025-01-21T01:05:00Z
dc.date.available2025-01-21T01:05:00Z
dc.date.issued2008
dc.description.abstractIn Penicillium purpurogenum, the gene encoding endoxylanase B (xynB) is highly expressed by xylan and repressed by glucose at the transcriptional level. The promoter of this gene has a modular structure, with eight putative XlnR binding sites in tandem (XlnR module), and upstream from them, four putative CreA binding sites (CreA module). Promoter fragments containing different modules were inserted into a plasmid, pAN49-1, which contains a basal fungal promoter linked to a reporter gene (lacZ) and its expression was studied in vivo in Aspergillus nidulans. The XlnR module is able to trigger high beta-galactosidase activity in the presence of xylan, but the lack of most XlnR sites notoriously reduces this enzymatic activity. No enzyme induction is observed if the orientation of the promoter fragment is inverted. The presence of the CreA module is necessary for glucose repression when beta-galactosidase activity is previously induced by xylan. However, when transformant strains containing the XlnR module but lacking all CreA sites were grown in glucose without pre-induction in xylan, a low beta-galactosidase activity was observed compared with the same transformants grown in xylan. These results agree with a double-lock regulatory mechanism for both direct and indirect repression of xylanolytic genes by glucose.
dc.fuente.origenWOS
dc.identifier.doi10.1007/s00294-008-0205-y
dc.identifier.eissn1432-0983
dc.identifier.issn0172-8083
dc.identifier.urihttps://doi.org/10.1007/s00294-008-0205-y
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95778
dc.identifier.wosidWOS:000258672800003
dc.issue.numero3
dc.language.isoen
dc.pagina.final141
dc.pagina.inicio133
dc.revistaCurrent genetics
dc.rightsacceso restringido
dc.subjectpromoter fragments
dc.subjectCreA sites
dc.subjectXlnR sites
dc.subjectin vivo expression
dc.subjectPenicillium purpurogenum
dc.subjectAspergillus nidulans
dc.subject.ods07 Affordable and Clean Energy
dc.subject.ods12 Responsible Consumption and Production
dc.subject.odspa07 Energía asequible y no contaminante
dc.subject.odspa12 Producción y consumo responsable
dc.titleFunctional analysis of the endoxylanase B (<i>xynB</i>) promoter from <i>Penicillium purpurogenum</i>
dc.typeartículo
dc.volumen54
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas