ENDOTHELIN-1 (ET)-INDUCED MOBILIZATION OF INTRACELLULAR CA2+ STORES FROM THE SMOOTH-MUSCLE FACILITATES SYMPATHETIC COTRANSMISSION BY POTENTIATION OF ADENOSINE 5'-TRIPHOSPHATE (ATP) MOTOR-ACTIVITY - STUDIES IN THE RAT VAS-DEFERENS

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dc.contributor.authorDONOSO, MV
dc.contributor.authorMONTES, CG
dc.contributor.authorLEWIN, J
dc.contributor.authorFOURNIER, A
dc.contributor.authorCALIXTO, JB
dc.contributor.authorHUIDOBROTORO, JP
dc.date.accessioned2025-01-23T19:22:05Z
dc.date.available2025-01-23T19:22:05Z
dc.date.issued1992
dc.description.abstractEndothelin-1 (ET) enhances nerve-stimulated contractions in epididymal (E) and prostatic (P) halves of the rat vas deferens, in addition to raising the basal tone in E. Whereas the peak increase in basal tone occurs in about 30 s, the maximal enhancement of neuro-transmission is observed within 5 min. The latter effect is long lasting is maintained even after extensive tissue washout. Furthermore, ET potentiates, in a concentration-dependent fashion, the adenosine 5'-triphosphate (ATP) or the adenylylimido-diphosphate (AMP-PNP) but not the noradrenaline (NA)-induced motor activity. The ATP motor response is partially blocked in media without Ca2+ plus 0.1 mM EGTA or following tissue incubation in buffer containing 10-50 nM nifedipine. However, these procedures do not modify significantly the ET-induced potentiation of the ATP contractions. The ET-induced potentiation of the ATP motor response is not modified by tissue preincubation in Ca2+-free buffer plus 10-30-mu-m ryanodine or 5-20 mM caffeine. The ET-induced rise in E basal tension is significantly reduced in the absence of external Ca2+ or by nifedipine; ryanodine does not modify this effect. Surgical denervation of the tissues does not obliterate the ET-induced potentiation of the ATP motor responses nor the ET increase in E basal tension in tissues superfused in Ca2+-free media or buffer with 2.5 mM Ca2+. Endothelin-1 does not significantly modify the overflow of H-3-NA, following transmural electrical depolarization of tissue nerve terminals. Hoe 140 did not interfere with the ET activity.
dc.fuente.origenWOS
dc.identifier.issn0196-9781
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/98958
dc.identifier.wosidWOS:A1992JL83400029
dc.issue.numero4
dc.language.isoen
dc.pagina.final840
dc.pagina.inicio831
dc.revistaPeptides
dc.rightsacceso restringido
dc.subjectENDOTHELIN
dc.subjectATP MOTOR ACTIVITY
dc.subjectCOTRANSMISSION
dc.subjectRAT VAS DEFERENS
dc.subjectENDOTHELIN-INDUCED ATP POTENTIATION
dc.subjectNIFEDIPINE
dc.subjectRYANODINE
dc.subjectCAFFEINE
dc.subjectENDOTHELIN AND CA2+ FLUXES
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleENDOTHELIN-1 (ET)-INDUCED MOBILIZATION OF INTRACELLULAR CA2+ STORES FROM THE SMOOTH-MUSCLE FACILITATES SYMPATHETIC COTRANSMISSION BY POTENTIATION OF ADENOSINE 5'-TRIPHOSPHATE (ATP) MOTOR-ACTIVITY - STUDIES IN THE RAT VAS-DEFERENS
dc.typeartículo
dc.volumen13
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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