Combined multi-omics and physiological approaches to elucidate drought-response mechanisms of durum wheat

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dc.catalogadorcarga
dc.contributor.authorArriagada Lagos Osvin
dc.contributor.authorMeneses, Claudio
dc.contributor.authorPedreschi, Romina
dc.contributor.authorNunez-Lillo, Gerardo
dc.contributor.authorMaureira, Carlos
dc.contributor.authorReveco Miranda Samantha Teresa
dc.contributor.authorVillarroel, Valentina
dc.contributor.authorSteinfort Needham Ursula Carla
dc.contributor.authorAlbornoz, Francisco
dc.contributor.authorCabas-Luhmann, Patricia
dc.contributor.authorSilva, Manuela
dc.contributor.authorMatus Mora Ivan Alonso
dc.contributor.authorSchwember Neira Andres Ricardo
dc.date.accessioned2025-09-30T10:00:17Z
dc.date.available2025-09-30T10:00:17Z
dc.date.issued2025
dc.description.abstractIntroduction Durum wheat is the most important cereal in the Mediterranean regions, where drought negatively affects grain yield. Therefore, our objective was to perform a multi-omics and integration analysis in conjunction with physiological trials to improve our understanding of drought tolerance mechanisms of durum wheat.Methods Genome-wide association study (GWAS) for yield components was performed on a panel of 225 elite durum wheat genotypes evaluated in eight sites under irrigated and rainfed conditions. Based on physiological parameters (net photosynthesis, intracellular CO2 content, transpiration and stomatal conductance) and grain yield, contrasting genotypes (susceptible and tolerant) to drought were identified. A transcriptomic (RNA-seq), metabolomic and integration analyses were performed to identify genes and metabolites associated with tolerance in durum wheat.Results Nine marker-trait associations were detected across 8 environments, and they were grouped into three QTL clusters (QTL_2A_TGW/GPS.1, QTL_2A_TGW/GPS.2, and QTL_2B_TGW/GPS.1), explaining between 5.15% and 14.29% of the phenotypic variation. One drought tolerant (QUC 3678-2016) and one susceptible (BRESCIA) genotype were identified based on physiological parameters. RNA-seq analysis showed that the genes regulated were mainly enriched in processes such as response to salicylic acid, plant organ senescence, synthesis of secondary metabolites, and immune response. Metabolic analysis showed that drought increased the contents of amino acids, sugars, and organic acids. The integration analysis identified 30 genes and six metabolites in the root and 30 genes and 10 metabolites in leaves as the primary variables in the drought-tolerant genotype, in which L-Proline was an important metabolite that allowed differentiating those two contrasting genotypes. A WRKY transcription factor was also positioned on the stable QTL QTN_2A_TGW/GPS.1 associated with the GENE-1342_238 SNP marker.Discussion These results open an opportunity to use new biomarkers in durum wheat breeding programs to develop resilient and high-yielding cultivars and ensure food security under water deficit conditions.
dc.fuente.origenWOS
dc.identifier.doi10.3389/fpls.2025.1540179
dc.identifier.eissn1664-462X
dc.identifier.issn0717-0297
dc.identifier.scopusidSCOPUS_ID:105007007366
dc.identifier.urihttps://doi.org/10.3389/fpls.2025.1540179
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105825
dc.identifier.wosidWOS:001497947500001
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Meneses Araya, Claudio Antonio; S/I; 1246418
dc.information.autorucS/I; Arriagada Lagos Osvin; S/I; 1194520
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Reveco Miranda Samantha Teresa; S/I; 186776
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Steinfort Needham Ursula Carla; S/I; 1020220
dc.information.autorucPrograma de Estudios Generales College; Matus Mora Ivan Alonso; S/I; 1066337
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Schwember Neira Andres Ricardo; S/I; 67919
dc.language.isoen
dc.pagina.final96
dc.pagina.inicio85
dc.revistaFRONTIERS IN PLANT SCIENCE
dc.rightsregistro bibliográfico
dc.subject<italic>Triticum durum</italic> L.
dc.subjectproline
dc.subjectWRKY
dc.subjectQTL
dc.subjectGWAS
dc.subjectRNA-seq
dc.titleCombined multi-omics and physiological approaches to elucidate drought-response mechanisms of durum wheat
dc.typeartículo
dc.volumen16
sipa.codpersvinculados1246418
sipa.codpersvinculados1194520
sipa.codpersvinculados186776
sipa.codpersvinculados1020220
sipa.codpersvinculados1066337
sipa.codpersvinculados67919
sipa.indexScopus
sipa.indexWOS
sipa.trazabilidadWOS-SCOPUS;2025-09-30
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