THE PROTEOGLYCAN DECORIN IS SYNTHESIZED AND SECRETED BY DIFFERENTIATED MYOTUBES

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dc.contributor.authorBRANDAN, E
dc.contributor.authorFUENTES, ME
dc.contributor.authorANDRADE, W
dc.date.accessioned2025-01-23T19:22:36Z
dc.date.available2025-01-23T19:22:36Z
dc.date.issued1991
dc.description.abstractProteoglycans (PGs) are important components of the skeletal muscle extracellular matrix (ECM). Skeletal muscles are composed of muscle fibers and mononucleated cells. The latter are known to synthesize and secrete several PGs. Rat skeletal muscle ECM contains a chondrotin/dermatan sulfate PG which was immunoprecipitated by antibodies against rat decorin. The synthesis and secretion of PGs by a mouse cell line was analyzed during in vitro differentiation. PGs were characterized by biochemical and immunological techniques including immunocytolocalization experiments. At least three different PGs are synthesized and secreted by differentiated myotubes: a 220 to 460 kDa heparan sulfate, a 250 to 310 kDa chondroitin/dermatan sulfate, and a 75 to 130 kDa chondroitin/dermatan sulfate. This latter PG was specifically immunoprecipitated with antibodies against rat fibroblast decorin. Indirect immunocytolocalization analysis revealed that decorin was localized inside the cells, with a strong reaction around the nuclei. During differentiation the relative proportions of some PGs changed. Thus, a decrease in the relative proportion of the heparan sulfate PG was observed, whereas a significant increase in the relative proportion of decorin was detected. No change in the large chondroitin/dermatan PG was seen during the differentiation process. The possible cell sources of decorin found in rat skeletal muscle ECM are discussed.
dc.fuente.origenWOS
dc.identifier.issn0171-9335
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/99066
dc.identifier.wosidWOS:A1991GD44500003
dc.issue.numero2
dc.language.isoen
dc.pagina.final216
dc.pagina.inicio209
dc.revistaEuropean journal of cell biology
dc.rightsacceso restringido
dc.subjectPROTEOGLYCANS
dc.subjectMYOTUBES
dc.subjectDIFFERENTIATION
dc.subjectDECORIN
dc.subjectEXTRACELLULAR MATRIX
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleTHE PROTEOGLYCAN DECORIN IS SYNTHESIZED AND SECRETED BY DIFFERENTIATED MYOTUBES
dc.typeartículo
dc.volumen55
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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