HERPUD1 governs tumor cell mitochondrial function via inositol 1,4,5-trisphosphate receptor-mediated calcium signaling

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dc.contributor.authorParedes, Felipe
dc.contributor.authorNavarro-Marquez, Mario
dc.contributor.authorQuiroga, Clara
dc.contributor.authorJimenez-Gallegos, Danica
dc.contributor.authorYeligar, Samantha M.
dc.contributor.authorParra, Valentina
dc.contributor.authorMueller, Marioly
dc.contributor.authorChiong, Mario
dc.contributor.authorQuest, Andrew F. G.
dc.contributor.authorSan Martin, Alejandra
dc.contributor.authorLavandero, Sergio
dc.date.accessioned2025-01-20T17:10:35Z
dc.date.available2025-01-20T17:10:35Z
dc.date.issued2024
dc.description.abstractThe intricate relationship between calcium (Ca2+) homeostasis and mitochondrial function is crucial for cellular metabolic adaptation in tumor cells. Ca2+-initiated signaling maintains mitochondrial respiratory capacity and ATP synthesis, influencing critical cellular processes in cancer development. Previous studies by our group have shown that the homocysteine-inducible ER Protein with Ubiquitin-Like Domain 1 (HERPUD1) regulates inositol 1,4,5-trisphosphate receptor (ITPR3) levels and intracellular Ca2+ signals in tumor cells. This study explores the role of HERPUD1 in regulating mitochondrial function and tumor cell migration by controlling ITPR3-dependent Ca2+ signals.We found HERPUD1 levels correlated with mitochondrial function in tumor cells, with HERPUD1 deficiency leading to enhanced mitochondrial activity. HERPUD1 knockdown increased intracellular Ca2+ release and mitochondrial Ca2+ influx, which was prevented using the ITPR3 antagonist xestospongin C or the Ca2+ chelator BAPTA-AM. Furthermore, HERPUD1 expression reduced tumor cell migration by controlling ITPR3-mediated Ca2+ signals. HERPUD1-deficient cells exhibited increased migratory capacity, which was attenuated by treatment with xestospongin C or BAPTA-AM. Additionally, HERPUD1 deficiency led to reactive oxygen species -dependent activation of paxillin and FAK proteins, which are associated with enhanced cell migration.Our findings highlight the pivotal role of HERPUD1 in regulating mitochondrial function and cell migration by controlling intracellular Ca2+ signals mediated by ITPR3. Understanding the interplay between HERPUD1 and mitochondrial Ca2+ regulation provides insights into potential therapeutic targets for cancer treatment and other pathologies involving altered energy metabolism.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.freeradbiomed.2023.11.022
dc.identifier.eissn1873-4596
dc.identifier.issn0891-5849
dc.identifier.urihttps://doi.org/10.1016/j.freeradbiomed.2023.11.022
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/91120
dc.identifier.wosidWOS:001137633200001
dc.language.isoen
dc.pagina.final34
dc.pagina.inicio24
dc.revistaFree radical biology and medicine
dc.rightsacceso restringido
dc.subjectHERPUD1
dc.subjectMitochondria
dc.subjectTumoral cell
dc.subjectOxidative stress
dc.subjectIP3 receptor
dc.subjectCalcium
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleHERPUD1 governs tumor cell mitochondrial function via inositol 1,4,5-trisphosphate receptor-mediated calcium signaling
dc.typeartículo
dc.volumen211
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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