Metabolic engineering of Saccharomyces cerevisiae for neoxanthin production

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dc.article.number176
dc.catalogadorpva
dc.contributor.authorArenas Frigolett, Natalia
dc.contributor.authorCataldo von Bohlen, Vicente Francisco
dc.contributor.authorAgosin T., Eduardo
dc.date.accessioned2025-08-04T20:55:23Z
dc.date.available2025-08-04T20:55:23Z
dc.date.issued2025
dc.date.updated2025-08-03T00:08:48Z
dc.description.abstractBackground Xanthophylls, a subclass of oxygenated carotenoids, are highly valued for their wide range of applications in the food and pharmaceutical industries, particularly due to their antioxidant properties and potential health benefits. Among these, neoxanthin, a less studied xanthophyll, has demonstrated significant therapeutic potential, including antioxidant and anticancer activities. Neoxanthin is also the primary precursor for the synthesis of other valuable compounds, such as fucoxanthin and β-damascenone, which are important in the cosmetic and pharmaceutical sectors. Results In this study, we report the first heterologous production of neoxanthin in Saccharomyces cerevisiae through a combination of metabolic and enzyme engineering. First, a S. cerevisiae strain was engineered to produce neoxanthin by expressing genes from the β-carotene and violaxanthin biosynthesis pathways. Following this, the VDL1 gene from Phaeodactylum tricornutum, responsible for converting violaxanthin into neoxanthin, was expressed, resulting in the production of 0.18 mg/gDCW of neoxanthin. To further enhance production, a pulse-fed galactose strategy was employed during shake-flask growth, leading to a 2.5-fold increase in neoxanthin yield. Additionally, transmembrane peptides were incorporated into the yeast cells to improve the accumulation of carotenoids, generating an increase of 3.8-fold, achieving a final production of 0.7 mg/gDCW of neoxanthin. Conclusions This is the highest reported yield of neoxanthin produced by engineered microorganisms, and the strategies employed here have considerable potential for scaling up production of this carotenoid.
dc.fechaingreso.objetodigital2025-08-03
dc.format.extent15 páginas
dc.fuente.origenAutoarchivo
dc.identifier.citationMicrobial Cell Factories. 2025 Aug 01;24(1):176
dc.identifier.doi10.1186/s12934-025-02789-8
dc.identifier.issn1475-2859
dc.identifier.urihttps://doi.org/10.1186/s12934-025-02789-8
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/105116
dc.information.autorucEscuela de Ingeniería; Arenas Frigolett, Natalia; S/I; 221015
dc.information.autorucEscuela de Ingeniería; Cataldo von Bohlen, Vicente Francisco; S/I; 250078
dc.information.autorucEscuela de Ingeniería; Agosin T., Eduardo; 0000-0003-1656-150X; 99630
dc.issue.numero1
dc.language.isoen
dc.nota.accesocontenido completo
dc.publisherSpringer Nature
dc.revistaMicrobial Cell Factories
dc.rightsacceso abierto
dc.rights.holderThe Author(s)
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCarotenoid
dc.subjectXanthophyll
dc.subjectNeoxanthin
dc.subjectMetabolic engineering
dc.subjectSaccharomyces cerevisiae
dc.subject.ddc620
dc.subject.deweyIngenieríaes_ES
dc.subject.ods03 Good health and well-being
dc.subject.odspa03 Salud y bienestar
dc.titleMetabolic engineering of Saccharomyces cerevisiae for neoxanthin production
dc.typeartículo
dc.volumen24
sipa.codpersvinculados221015
sipa.codpersvinculados250078
sipa.codpersvinculados99630
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