Decoding the Hayward kiwi (Actinidia deliciosa var Hayward) genome: transcriptomic responses to drought and salinity and AdhSAP4’s role in salinity stress responses

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dc.article.number1637092
dc.catalogadorjca
dc.contributor.authorParra, Samuel
dc.contributor.authorNúñez-Lillo, Gerardo
dc.contributor.authorTapia-Reyes, Patricio
dc.contributor.authorCarrasco-Lozano, Emerson Clovis
dc.contributor.authorPorcile, Vincenzo
dc.contributor.authorGonzalez-Calquin, Christian
dc.contributor.authorAmaza, Leticia
dc.contributor.authorQuiroz, Luis Felipe
dc.contributor.authorMeneses Araya, Claudio Antonio
dc.contributor.authorHandford, Michael
dc.contributor.authorNorambuena, Lorena
dc.contributor.authorMartínez, Juan Pablo
dc.contributor.authorStange, Claudia
dc.date.accessioned2025-12-05T13:20:37Z
dc.date.available2025-12-05T13:20:37Z
dc.date.issued2025
dc.description.abstractAbiotic stresses such as drought and salinity pose major limitations to crop productivity, particularly in sensitive species like the Hayward kiwi (Actinidia deliciosa var. Hayward). Stress-associated proteins (SAPs), defined by conserved A20/AN1 zinc finger domains, are emerging as key modulators of plant stress responses. Despite their relevance in model plants, their functional roles in kiwi remain unexplored. In this study we assembled a high-quality haploid reference genome for Hayward kiwi, annotating 42,797 protein-coding genes. RNA-Seq profiling of in vitro leaves exposed to drought (20% PEG-6000) and salinity (200 mM NaCl) at 6 and 24 hours identified differentially expressed genes (DEGs). The differential gene expression kinetics between drought and salinity suggest distinct adaptation mechanisms. Fourteen SAP genes were identified, with AdhSAP4 showing salt-induced expression and homology to rice OsSAP7. Nuclear localization of AdhSAP4-GFP was confirmed, and transgenic tobacco lines overexpressing AdhSAP4 exhibited heightened salt sensitivity, reduced growth, and chlorophyll loss. This study establishes a genomic and transcriptomic framework for kiwi stress biology and confirms AdhSAP4 as a negative regulator of salinity tolerance. The evolutionary conservation of SAP function highlights their potential as biotechnological targets for enhancing stress resilience in perennial crops like kiwi.
dc.fechaingreso.objetodigital2025-12-05
dc.format.extent17 páginas
dc.fuente.origenSIPA
dc.identifier.doi10.3389/fpls.2025.1637092
dc.identifier.issn1664-462X
dc.identifier.urihttps://doi.org/10.3389/fpls.2025.1637092
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/107287
dc.identifier.wosidWOS:001587814700001
dc.information.autorucFacultad de Agronomía e Ingenieria Forestal; Meneses Araya, Claudio Antonio; 0000-0002-6452-8950; 1246418
dc.language.isoen
dc.nota.accesocontenido completo
dc.revistaFrontiers in Plant Science
dc.rightsacceso abierto
dc.rights.licenseAttribution License (CC BY)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHayward kiwi (Actinidia deliciosa)
dc.subjectSalinity stress
dc.subjectDrought stress
dc.subjectTranscriptomic (RNAseq)
dc.subjectGenome assembly
dc.subjectStress associated protein (SAP)
dc.subject.ddc300
dc.subject.ods14 Life below water
dc.subject.ods02 Zero hunger
dc.subject.odspa14 Vida submarina
dc.subject.odspa02 Hambre cero
dc.titleDecoding the Hayward kiwi (Actinidia deliciosa var Hayward) genome: transcriptomic responses to drought and salinity and AdhSAP4’s role in salinity stress responses
dc.typeartículo
dc.volumen16
sipa.codpersvinculados1246418
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