Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen <i>Piscirickettsia salmonis</i>

Simple item page
dc.contributor.authorWilhelm, V
dc.contributor.authorSoza, C
dc.contributor.authorMartínez, R
dc.contributor.authorRosemblatt, M
dc.contributor.authorBurzio, LO
dc.contributor.authorValenzuela, PDT
dc.date.accessioned2025-01-21T01:07:17Z
dc.date.available2025-01-21T01:07:17Z
dc.date.issued2005
dc.description.abstractWe have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
dc.fuente.origenWOS
dc.identifier.eissn0717-6287
dc.identifier.issn0716-9760
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/96304
dc.identifier.wosidWOS:000229899100009
dc.issue.numero1
dc.language.isoen
dc.pagina.final82
dc.pagina.inicio69
dc.revistaBiological research
dc.rightsacceso restringido
dc.subjectPiscirickettsia salmonis
dc.subjectHsp60
dc.subjectHsp70
dc.subjectgroEL
dc.subjectdnaK
dc.subjectimmune response
dc.subject.ods03 Good Health and Well-being
dc.subject.odspa03 Salud y bienestar
dc.titleProduction and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen <i>Piscirickettsia salmonis</i>
dc.typeartículo
dc.volumen38
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
Files
Collections
Artículos de revistas