Proteomic analysis and identification of copper stress-regulated proteins in the marine alga <i>Scytosiphon gracilis</i> (Phaeophyceae)

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dc.contributor.authorContreras, Loretto
dc.contributor.authorMoenne, Alejandra
dc.contributor.authorGaillard, Fanny
dc.contributor.authorPotin, Philippe
dc.contributor.authorCorrea, Juan A.
dc.date.accessioned2025-01-21T00:06:49Z
dc.date.available2025-01-21T00:06:49Z
dc.date.issued2010
dc.description.abstractA proteomic analysis combining peptide de novo sequencing and BLAST analysis was used to identify novel proteins involved in copper tolerance in the marine alga Scytosiphon gracilis (Phaeophyceae). Algal material was cultivated in seawater without copper (control) or supplemented with 100 mu g L(-1) for 4 days, and protein extracts were separated by two-dimensional gel electrophoresis (2-DE). From the proteins obtained in the copper treatment, 25 over-expressed, 5 under-expressed and 5 proteins with no changes as compared with the control, were selected for sequencing. Tryptic-peptides obtained from 35 spots were analyzed by capillary liquid chromatography and tandem mass spectroscopy (capLC/MS/MS). and protein identity was determined by BLASTP. We identified 19 over-expressed proteins, including a chloroplast peroxiredoxin, a cytosolic phosphomannomutase, a cytosolic glyceraldehyde-3-phosphate dehydrogenase, 3 ABC transporters, a chaperonine, a subunit of the proteasome and a tRNA synthase, among others. The possible involvement of these over-expressed proteins in buffering oxidative stress and avoiding metal uptake in S. gracilis exposed to copper excess is discussed taking into consideration the information available for other plant models. (C) 2009 Elsevier B.V. All rights reserved.
dc.fuente.origenWOS
dc.identifier.doi10.1016/j.aquatox.2009.10.007
dc.identifier.issn0166-445X
dc.identifier.urihttps://doi.org/10.1016/j.aquatox.2009.10.007
dc.identifier.urihttps://repositorio.uc.cl/handle/11534/95604
dc.identifier.wosidWOS:000274978500001
dc.issue.numero2
dc.language.isoen
dc.pagina.final89
dc.pagina.inicio85
dc.revistaAquatic toxicology
dc.rightsacceso restringido
dc.subjectCopper stress
dc.subjectPhaeophyceae
dc.subjectProteomics
dc.subjectScytosiphon
dc.subjectSeaweeds
dc.subject.ods14 Life Below Water
dc.subject.ods15 Life on Land
dc.subject.ods13 Climate Action
dc.subject.odspa14 Vida submarina
dc.subject.odspa15 Vida de ecosistemas terrestres
dc.subject.odspa13 Acción por el clima
dc.titleProteomic analysis and identification of copper stress-regulated proteins in the marine alga <i>Scytosiphon gracilis</i> (Phaeophyceae)
dc.typeartículo
dc.volumen96
sipa.indexWOS
sipa.trazabilidadWOS;2025-01-12
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